BACKGROUND Pulse frequency is one of the key determinants of analgesic outcomes by transcutaneous electrical nerve stimulation (TENS). However, optimal settings remain unclear. OBJECTIVE To compare the effects of different frequencies of TENS, on pressure pain threshold and tolerance. METHODS Currents with pulse duration of 110 μs, and pulse frequencies of 60 pps or 150 pps were applied on the volar aspects of the dominant forearms of 20 healthy volunteers, on two consecutive days, in a randomized order. Threshold and tolerance were measured at the beginning, after the 15th and 30th minutes of the applications, and 30 minutes after the applications. RESULTS Pressure pain threshold and tolerance values were higher at the 150 pps frequency, at all measurement times (p< 0.05). However, no frequencyXtime interaction and time-dependent changes were found for the outcome measures (p> 0.05). CONCLUSIONS These findings established that, at 150 pps conventional TENS, threshold and tolerance values were consistently higher. These results are presented to inform future research regarding optimal conventional TENS parameters and to provide support for clinical applications.