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The HIV-1 Rev protein mediates export of unspliced and singly spliced viral transcripts by binding to the Rev response element (RRE) and recruiting the cellular export factor CRM1. Here, we investigated the recruitment of Rev to the transcription sites of HIV-1 reporters that splice either post- or cotranscriptionally. In both cases, we observed that Rev localized to the transcription sites of the reporters and recruited CRM1. Rev and CRM1 remained at the reporter transcription sites when cells were treated with the splicing inhibitor Spliceostatin A (SSA), showing that the proteins associate with RNA prior to or during early spliceosome assembly. Fluorescence recovery after photobleaching (FRAP) revealed that Rev and CRM1 have similar kinetics as the HIV-1 RNA, indicating that Rev, CRM1, and RRE-containing RNAs are released from the site of transcription in one single export complex. These results suggest that cotranscriptional formation of a stable export complex serves as a means to ensure efficient export of unspliced viral RNAs.
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
April 1996,
Proceedings of the National Academy of Sciences of the United States of America,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
August 2014,
eLife,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
March 1999,
Molecular cell,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
October 1995,
Journal of molecular biology,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
November 2010,
PLoS genetics,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
June 2012,
Biochimica et biophysica acta,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
January 2008,
Medecine sciences : M/S,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
July 2011,
Journal of molecular biology,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
March 2008,
Protein science : a publication of the Protein Society,
Isabel Nawroth, and
Florian Mueller, and
Eugenia Basyuk, and
Nancy Beerens, and
Ulrik L Rahbek, and
Xavier Darzacq, and
Edouard Bertrand, and
Jørgen Kjems, and
Ute Schmidt
September 2021,
The Journal of cell biology,
