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Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon : II. A new Medium for the production of shikonin derivatives. 1981
Research Center, Mitsui Petrochemical Industries, Ltd., 6-1-2, Waki-cho, Kuga-gun, 740, Yamaguchi-ken, Japan.
An excellent new medium was developed for the production of shikonin derivatives by suspension cultures of Lithospermum erythrorhizon. We investigated the effects of all the components of White's medium on the production of these derivatives. Nitrate, phosphate, copper, sulfate and sucrose had especially marked effects. With the new, M-9, medium produced from these studies the yield of shikonin derivatives was 1400 mg/l and the yield for dried cells was about 12%, whereas it was 120 mg/l, or about 2% with White's medium.
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