The identification of fiber types in skeletal muscle: a continual dilemma. 1986

P D Gollnick, and D R Hodgson

We have attempted to present an overview of some of the methods used for identifying and studying the fibers and motor units of skeletal muscle, and to give a short discourse on the particular strengths and weaknesses of these methods. It was not our intention to reach a final conclusion of there being only one way to identify muscle fibers or that the subject is closed to additional research. Quite the contrary, we intended to demonstrate that in the past two decades there has been an explosion of knowledge in this field. In the introduction, we suggested that the era of a simple classification scheme for categorizing fibers has passed. We also posed a number of questions. It seems that an appropriate conclusion would be to return to these questions with some answers. Our first question was "what is the purpose of fiber typing?" To us, its intent is to provide as much information as possible about fibers, accentuating their similarities and differences, such that the designated groupings are most meaningful. This has become increasingly difficult as the complex nature of skeletal muscle fibers has been unravelled and the diversity of those fibers previously thought to be similar is exposed. However, fiber typing schemes provide valuable information concerning the nature of the fibers and are important in attempting to describe the nature of muscle. Therefore, fiber typing is a necessary step in describing the complex characteristics of skeletal muscle. We also asked "is more information needed?" Clearly more information is needed to develop a complete understanding of the biochemical and functional aspects of muscle and how it adapts to a variety of experimental or naturally occurring perturbations. Thus, as time passes and more information is amassed, a constant revision of the schemes for identifying fibers can be expected. This is a positive sign of a viable field. A third question was "is it worth trying to keep up or can one be expected to keep up with the seemingly rapid changes in the identification schemes used for fiber typing?" This seems like an absurd question, and yet, it has been posed to us by university professors who are "comfortable with" and continue to use the old system(s) of muscle and fiber identification and who do not want to "see things change." This question, when asked in any field, implies an unwillingness to make the effort to stay abreast with advancements occurring in that field.(ABSTRACT TRUNCATED AT 400 WORDS)

UI MeSH Term Description Entries
D008870 Microtubules Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS. Microtubule
D008931 Mitochondria, Muscle Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available. Sarcosomes,Mitochondrion, Muscle,Muscle Mitochondria,Muscle Mitochondrion,Sarcosome
D009046 Motor Neurons Neurons which activate MUSCLE CELLS. Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D009119 Muscle Contraction A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments. Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D009124 Muscle Proteins The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN. Muscle Protein,Protein, Muscle,Proteins, Muscle
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D009210 Myofibrils The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES . Myofilaments,Myofibril,Myofilament
D003584 Cytological Techniques Methods used to study CELLS. Cytologic Technics,Cytological Technic,Cytological Technics,Cytological Technique,Technic, Cytological,Technics, Cytological,Technique, Cytological,Techniques, Cytological,Cytologic Technic,Technic, Cytologic,Technics, Cytologic
D006651 Histocytochemistry Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods. Cytochemistry
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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