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A simultaneous quantitative HPLC assay was developed for tiaramide hydrochloride (4-[(5-chloro-2-oxo-3(2H)-benzothiazolyl) acetyl]-1-piperazine ethanol hydrochloride) and its main metabolites, namely, 1 - [ (5-chloro-2-oxo-3 (2H) -benzothiazolyl) -acetyl]-piperazine (DETR) and 4-[(5-chloro-2-oxo-3(2H)-benzothiazolyl) acetyl] -piperazine acetic acid (TRAA). The assay was employed to determine plasma levels of tiaramide and its main metabolite, TRAA, after intravenous administration of tiaramide hydrochloride to rabbits.
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