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The 3A33 monoclonal antibody, obtained by fusing rat immune lymphocytes with mouse plasmacytoma cells, was directed against mouse macrophages. Antibody 3A33, a rat IgG2a, reacted with macrophages from all the mouse strains tested, with mouse blood monocytes and with 56% of bone marrow cells, but not with T lymphocytes. It immunoprecipitated an antigen with alpha and beta subunits, found to be identical to Mac-1 antigen after cross-absorption experiments with M1/70 monoclonal antibody. The two antigenic determinants of the Mac-1 molecule identified by the 3A33 and M1/70 antibodies both displayed reduced expression on inflammatory macrophages and comparable resistance to trypsin digestion. The sites of the determinants on this molecule seemed close together judging from the ability of both the 3A33 and M1/70 antibodies to block C3bi receptor sites and compete for cell binding. However, unlike antibody M1/70, 3A33 never reacted with human cells bearing Mac-1 antigen. Therefore, two closely related epitopes of the Mac-1 molecule - one specific for mouse and one common to mouse and man, were recognized by these monoclonal antibodies.
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
October 1997,
Infection and immunity,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
October 1985,
Molecular immunology,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
October 1987,
Microbial pathogenesis,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
September 1988,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
May 1987,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
April 1979,
European journal of immunology,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
April 1982,
Science (New York, N.Y.),
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
December 1988,
Cancer research,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
May 1985,
Brain research,
A Martin, and
R Le Corre, and
P Pellen, and
D Bourel, and
G Merdrignac, and
B Genetet, and
L Toujas
November 1978,
Proceedings of the National Academy of Sciences of the United States of America,
