Quantitative, reader-based lateral flow technology is utilized for determination of ochratoxin contamination levels in wheat by the QuickTox Kit for QuickScan Ochratoxin-A. Naturally contaminated wheat samples were used to challenge the assay in the range of 0-100 ppb in linearity, selectivity, robustness, and stability, and in internal and external matrix studies. Performance was judged against criteria established by the AOAC Research Institute Performance Tested Method program prior to beginning the validation studies. All data produced during this work conformed to the acceptance criteria. Linear dose responses with R2 exceeding 0.97 and RSDr values between 6.22 and 17.10% for positive samples were observed in linearity and internal and external matrix studies. Ochratoxin A (OTA) and ochratoxin B (OTB) were detected by the assay. Assay sensitivity towards OTB was approximately 50% relative to OTA detection. Other common mycotoxins did not affect assay results. Variations in assay timing, temperature, and sample volume encompassed in the robustness study did not yield results outside the acceptable range. Determination of ochratoxin in wheat is facilitated using the QuickTox Kit for QuickScan Ochratoxin-A kit.