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An automatized technique to count reticulocytes by means of flow cytometry is described. Blood samples were stained by the fluorescent dye acridine orange without the use of fixative. Scatter and red fluorescence of the blood cells were measured in a flow cytometer. A discrimination between reticulocytes and erythrocytes was only achieved by using logarithmic amplification. The discrimination was better in peak mode than in area mode. The optimum dye concentration was 0.5 mg/liter acridine orange. At lower dye concentrations, not all reticulocytes were measured, whereas at higher dye concentrations the degree of discrimination between reticulocytes and erythrocytes decreased. There was a suitable discrimination between reticulocytes and erythrocytes. The reticulocyte numbers were scored by flow cytometry as well as by microscope for blood samples with 0.1-14% reticulocytes. The correlation between both methods was close.
F J Schmitz, and
E Werner
March 1993,
Annals of hematology,
F J Schmitz, and
E Werner
November 1992,
Veterinary pathology,
F J Schmitz, and
E Werner
May 1988,
Journal of immunological methods,
F J Schmitz, and
E Werner
December 1997,
Osaka city medical journal,
F J Schmitz, and
E Werner
January 1993,
Acta haematologica,
F J Schmitz, and
E Werner
February 2008,
Experimental hematology,
F J Schmitz, and
E Werner
February 1992,
Scandinavian journal of clinical and laboratory investigation,
F J Schmitz, and
E Werner
August 2007,
Clinica chimica acta; international journal of clinical chemistry,
F J Schmitz, and
E Werner
May 1997,
Mutation research,
F J Schmitz, and
E Werner
January 2005,
Methods in molecular biology (Clifton, N.J.),
