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A murine monoclonal antibody (MAb) was obtained that showed unique specificity for the immunizing T-cell line HPB-ALL. This antibody, C37 (an IgG1,K) also reacted with a small (2-5%) population of normal peripheral blood T (PBL-T) cells. These C37-positive (C37+) cells were found in both the T4/Leu3+ and T8/Leu2+ subsets. Like OKT3 antibody, C37 induced T-cell mitogenesis with a peak proliferative response at day 3. In long-term cultures containing irradiated autologous feeder cells and IL-2, C37 antibody caused the selective expansion of C37+ T cells. On HPB-ALL cells C37 induced comodulation of the T3 molecule. C37 precipitated a disulfide-linked dimer characteristic of the T-cell antigen receptor consisting of an alpha-subunit (45-48 kD) and a beta-subunit (38-42 kD) from both C37+ T-cell blasts of a normal individual and HPB-ALL cells that were surface radioiodinated. However, the precipitated molecule isolated from C37 antibody-activated T-cell blasts exhibited a different pI from that isolated from HPB-ALL cells. Our studies indicate that C37 recognizes an epitope on the T-cell receptor molecule that is shared by a subpopulation of human T cells, which raises the possibility that multiple variable-region associated and/or framework-like determinants of the T-cell antigen receptor can be defined serologically and used in functional and molecular studies of T-cell subsets.
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
November 1992,
Biulleten' eksperimental'noi biologii i meditsiny,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
January 1989,
Immunogenetics,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
June 1985,
The Journal of experimental medicine,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
July 1985,
European journal of immunology,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
August 1994,
Hybridoma,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
January 1991,
Microbiology and immunology,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
October 1986,
Proceedings of the National Academy of Sciences of the United States of America,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
January 1991,
Journal of immunology (Baltimore, Md. : 1950),
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
February 1993,
Fukuoka igaku zasshi = Hukuoka acta medica,
C Y Wang, and
Y Bushkin, and
R Pica, and
C Lane, and
H McGrath, and
D N Posnett
January 1991,
Allergie und Immunologie,
