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The intestinal microvillar enzyme complex sucrase-isomaltase has been studied in cystic fibrosis and control ileum. A number of biochemical parameters of the enzyme in ileum homogenates have been determined. Both solubilized as well as membrane-bound sucrase-isomaltase were analyzed with respect to their reaction with monoclonal antibodies against human sucrase-isomaltase. Finally the subcellular localization of sucrase-isomaltase was verified by immunoelectronmicroscopy or via the analysis of purified brush-border membrane preparations. At all levels no significant differences could be detected between sucrase-isomaltase of cystic fibrosis and control ileum. It is concluded that an abnormal subcellular localization and/or abnormal enzymatic activity of sucrase-isomaltase in cystic fibrosis intestine cannot explain the markedly decreased disaccharidase activities in amniotic fluids from pregnancies resulting in a child affected with cystic fibrosis.
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
July 1986,
The Biochemical journal,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
October 1975,
The New England journal of medicine,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
October 1976,
The New England journal of medicine,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
July 1995,
Journal of pediatric gastroenterology and nutrition,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
February 1974,
Lancet (London, England),
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
November 1982,
FEBS letters,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
April 1982,
The Journal of biological chemistry,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
March 2017,
Biochimica et biophysica acta. Molecular basis of disease,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
February 2024,
Biochimica et biophysica acta. Molecular basis of disease,
H J Sips, and
A H Claass, and
J M van Dongen, and
R Willemsen, and
A T Hoogeveen, and
H Galjaard, and
M Sinaasappel, and
H P Hauri, and
E E Sterchi
January 2001,
Biochimica et biophysica acta,
