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Single particle tracking (SPT) of transmembrane receptors in the plasma membrane often reveals heterogeneous diffusion. A thorough interpretation of the displacements requires an extensive analysis suited for discrimination of different motion types present in the data. Here the diffusion pattern of the homomeric alpha3-containing glycine receptor (GlyR) is analyzed in the membrane of HEK 293 cells. More specifically, the influence of the alpha3 RNA splice variants alpha3K and alpha3L on lateral membrane diffusion of the receptor is revealed in detail. Using a combination of ensemble and local SPT analysis, free and anomalous diffusion parameters are determined. The GlyR alpha3 free diffusion coefficient is found to be 0.13 +/- 0.01 microm2/s and both receptor variants display confined motion. The confinement probability level and residence time are significantly elevated for the alpha3L variant compared to the alpha3K variant. Furthermore, for the alpha3L GlyR, the presence of directed motion was also established, with a velocity matching that of saltatory vesicular transport. These findings reveal that alpha3 GlyRs are prone to different types of anomalous diffusion and reinforce the role of RNA splicing in determining lateral membrane trafficking.
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
January 1997,
Annual review of biophysics and biomolecular structure,
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
January 2012,
Methods in enzymology,
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
January 2015,
Small (Weinheim an der Bergstrasse, Germany),
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
September 1995,
Biophysical journal,
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
January 2018,
Methods in molecular biology (Clifton, N.J.),
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
January 2015,
Methods in molecular biology (Clifton, N.J.),
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
September 2009,
The European journal of neuroscience,
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
May 2024,
Discover nano,
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
June 2013,
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada,
Kristof Notelaers, and
Susana Rocha, and
Rik Paesen, and
Nick Smisdom, and
Ben De Clercq, and
Jochen C Meier, and
Jean-Michel Rigo, and
Johan Hofkens, and
Marcel Ameloot
November 2009,
Bulletin of mathematical biology,
