Biomaterial Database

Resolution and identification of O-phosphoserine, O-phosphothreonine, O-phosphotyrosine, and gamma-carboxyglutamic acid as their fluorescent o-phthalaldehyde derivatives by high performance liquid chromatography. 1986

R W Etheredge, and M J Glimcher

High performance liquid chromatography was used to resolve O-phosphoserine, O-phosphothreonine, and O-phosphotyrosine as their fluorescent o-phthalaldehyde derivatives. By adjusting the buffer system, very small amounts of O-phosphothreonine could be detected and quantitated in the presence of very large amounts of O-phosphoserine. In addition, gamma-carboxyglutamic acid and glutamic acid were also separated and quantitated. Depending on the buffer used, various combinations of these amino acids could be resolved in a single run.

UI	MeSH Term	Description	Entries
D009764	o-Phthalaldehyde	A reagent that forms fluorescent conjugation products with primary amines. It is used for the detection of many biogenic amines, peptides, and proteins in nanogram quantities in body fluids.	ortho-Phthalaldehyde,Orthophthaldialdehyde,o-Phthaldialdehyde,ortho-Phthalic Aldehyde,Aldehyde, ortho-Phthalic,o Phthalaldehyde,o Phthaldialdehyde,ortho Phthalaldehyde,ortho Phthalic Aldehyde
D010768	Phosphoserine	The phosphoric acid ester of serine.	Serine Phosphate,Phosphorylserine,Seryl Phosphate,Phosphate, Serine,Phosphate, Seryl
D010769	Phosphothreonine	The phosphoric acid ester of threonine. Used as an identifier in the analysis of peptides, proteins, and enzymes.	Threonine Phosphate,Phosphate, Threonine
D002851	Chromatography, High Pressure Liquid	Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.	Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012694	Serine	A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.	L-Serine,L Serine
D013912	Threonine	An essential amino acid occurring naturally in the L-form, which is the active form. It is found in eggs, milk, gelatin, and other proteins.	L-Threonine,L Threonine
D014443	Tyrosine	A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.	L-Tyrosine,Tyrosine, L-isomer,para-Tyrosine,L Tyrosine,Tyrosine, L isomer,para Tyrosine
D015055	1-Carboxyglutamic Acid	Found in various tissues, particularly in four blood-clotting proteins including prothrombin, in kidney protein, in bone protein, and in the protein present in various ectopic calcifications.	gamma-Carboxyglutamate,gamma-Carboxyglutamic Acid,3-Amino-1,1,3-propanetricarboxylic Acid,1 Carboxyglutamic Acid,gamma Carboxyglutamate,gamma Carboxyglutamic Acid