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Fractionated Photosystem (PS) I particles consisting of six, five or two core proteins were analyzed by HPLC for chlorophyll (Chl) a' and phylloquinone (PhQ). Each particle had a Chl a/P700 molar ratio of 50-55 and contained ca. 2 molecules of Chl a' per P700. Deliberate control of eluent composition led to isolated elution of PhQ and β-carotene in the normal-phase chromatogram. Based on these a simple HPLC procedure has been established to determine the PhQ/P700 molar ratio, which was ca. 2 for the larger two PS I particles and ca. 1 for the smallest particle, in line with previous reports.
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