[The effect of the cell separation procedure on neutrophilic granulocyte function. I. Research on the morphology and phagocytosis behavior of isolated granulocytes]. 1986

R Lehmann, and M Lehmann, and S Preussner, and G Hess

The investigations were aimed at detecting the impact of various cell separating techniques on the morphology and function of neutrophilic granulocytes. Methodically simple cell separating techniques were used, such as spontaneous sedimentation of ACD-AG blood and mechanically defibrinated blood and a flotation technique which makes use of cell isolation by means of a separating solution and centrifugation. Granulocytes which were isolated by the cell separator Haemonetics M 30 for therapeutic purposes and which, according to data in literature, are not damaged by cell separating techniques were used as reference cells. The electronic-optical evaluation of granulocyte morphology and the in vitro investigation of the phagocytic capacity for rice starch (ingestion rate) of differently isolated granulocytes were made after cell separation.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D010587 Phagocytosis The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES). Phagocytoses
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D006098 Granulocytes Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS. Granulocyte
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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