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A panel of monoclonal antibodies (mAbs) against human thyroglobulin (hTg) was obtained by somatic fusion of the nonsecreting myeloma cell line P3X66 Ag8/0 and spleen cells of Balb/c mice immunized with purified hTg. Antibody secreting clones were selected by solid phase enzyme immunoassay and analyzed for cross-reaction with Tg from several animal species. Twelve out of 15 mAbs cross-reacted with both rat and mouse Tg and 11 Mabs cross-reacted with bovine Tg. The cross-reaction with mouse Tg paralleled that of rat Tg, whereas discrete differences between the cross-reactivity patterns with bovine Tg were observed. Two clones secreted mAbs specific for hTg. We further characterized the mAbs and found that three mAbs recognized T4-containing determinants and one mAb reacted with both T4 and T3-containing determinants on the Tg molecule. The binding of the mAbs to hormonogenic determinants depended upon the thyroid hormone content of the molecule and the integrity of the three dimensional structure of Tg. One other mAb reacted with four peptides of CNBr-cleaved hTg, indicating the recognition of a repetitive determinant in the hTg molecule distinct from the hormonogenic regions.
M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
July 1997,
Microbiology (Reading, England),
M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
February 1988,
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M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
December 1992,
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M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
January 1983,
Annals of the New York Academy of Sciences,
M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
April 1997,
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M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
January 1985,
Journal of interferon research,
M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
January 1983,
Basic life sciences,
M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
December 1983,
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M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
July 1984,
Federation proceedings,
M H De Baets, and
R Theunissen, and
K Kok, and
J J de Vijlder, and
P J van Breda Vriesman
February 1990,
FEBS letters,
