An autoradiographic technique was used to detect changes in ornithine decarboxylase in rat dorsal root ganglion neurons after sciatic nerve lesions. Binding of [3H]difluoromethylornithine ([3H]DFMO) to tissue sections of L5 ganglia was compared between axotomized and unoperated ganglia at 0.5, 1, 2, 3, 4, 5, 7, 8, 9, 11, 14, and 30 days after a crush lesion of the sciatic nerve. The [3H]DFMO binding to axotomized ganglion neurons was elevated compared with the unoperated side at 0.5, 1, 5, 7, 8, and 11 days postoperation. Enzymatic measurements of ornithine decarboxylase on ganglia at 1, 4, 8, and 9 days after sciatic nerve crush confirmed basic patterns of enzyme activity comparisons derived from autoradiography. Compared with patterns of [3H]actinomycin D binding to nuclei during the same periods after axotomy, [3H]DFMO binding increased prior to increases in [3H]actinomycin D binding within the first week. After that time, changes in both variables occurred in parallel. The data suggest that increased activities of ornithine decarboxylase or concentrations of polyamines may be linked to the induction of increased RNA synthesis in the early phases of the axon reaction.