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We have examined the genetic basis for the expression of a human cross-reactive idiotype (CRI) commonly found on monoclonal IgM rheumatoid factors. The CRI was identified with a monoclonal antibody (17.109) and has been localized previously to the kappa-variable region. By using the human lymphoblastoid cell line WI-L2-729-HF2, and mononuclear cells from several sources, a panel of hybridomas was generated that produced 17.109 CRI-positive Ig. A recently cloned human germ-line V kappa III gene, Humkv305, served as a probe to identify genes which were rearranged and expressed in 17.109 CRI-positive and -negative hybridomas. This probe, when hybridized to human genomic DNA under stringent conditions, identified only two to five germ-line bands. In 10 separate 17.109 CRI-positive hybridoma clones, an additional rearranged V kappa band was identified. The probe did not anneal to rearranged V kappa bands in hybridoma clones that produced kappa-chains lacking the CRI. RNA dot-blot studies provided evidence for expression of genes hybridizing to the Humkv305 probe. The results indicate that the 17.109 CRI is a serologic marker for a single V kappa gene, or a small family of closely related V kappa genes, which is identified by the Humkv305 probe.
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P J Chen, and
T J Kipps, and
G Starkebaum, and
J G Heitzmann, and
V Radoux, and
S Fong, and
D A Carson
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R D Goldfien, and
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T J Kipps, and
G Starkebaum, and
J G Heitzmann, and
V Radoux, and
S Fong, and
D A Carson
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R D Goldfien, and
P J Chen, and
T J Kipps, and
G Starkebaum, and
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D A Carson
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R D Goldfien, and
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G Starkebaum, and
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D A Carson
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R D Goldfien, and
P J Chen, and
T J Kipps, and
G Starkebaum, and
J G Heitzmann, and
V Radoux, and
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D A Carson
November 1991,
Journal of immunology (Baltimore, Md. : 1950),
R D Goldfien, and
P J Chen, and
T J Kipps, and
G Starkebaum, and
J G Heitzmann, and
V Radoux, and
S Fong, and
D A Carson
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R D Goldfien, and
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G Starkebaum, and
J G Heitzmann, and
V Radoux, and
S Fong, and
D A Carson
November 1980,
Journal of immunology (Baltimore, Md. : 1950),
R D Goldfien, and
P J Chen, and
T J Kipps, and
G Starkebaum, and
J G Heitzmann, and
V Radoux, and
S Fong, and
D A Carson
June 1990,
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