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Mice homozygous for the mutation myelin deficient (mld), an allele of shiverer, exhibit decreased CNS myelination, tremors, and convulsions of progressively increasing severity leading to an early death. In this report we demonstrate in mld mice that the gene encoding myelin basic protein (MBP) is expressed at decreased levels and on an abnormal temporal schedule relative to the wild-type gene. Southern blot analyses, field-inversion gel electrophoresis studies, and analyses of mld MBP cosmid clones indicate that there are multiple linked copies of the MBP gene in mld mice. We have introduced an MBP transgene into mld mice and found that myelination increases and tremors and convulsions decrease. Mld and shiverer mice with zero, one, or two copies of the MBP transgene express distinct levels of MBP mRNA and myelin. The availability of a range of mice expressing graded levels of myelin should facilitate quantitative analysis of the roles of MBP in the myelination process and of myelin in nerve function.
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
November 1984,
Developmental biology,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
January 1980,
Neuroscience,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
January 1989,
Schweizer Archiv fur Neurologie und Psychiatrie (Zurich, Switzerland : 1985),
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
May 2015,
Brain research,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
July 2004,
Journal of neuroscience research,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
October 1983,
Brain research,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
April 1998,
Journal of neuroimmunology,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
February 1987,
Journal of neurochemistry,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
February 1990,
The EMBO journal,
B Popko, and
C Puckett, and
E Lai, and
H D Shine, and
C Readhead, and
N Takahashi, and
S W Hunt, and
R L Sidman, and
L Hood
January 1984,
Neurochemical pathology,
