Biomaterial Database

[Distribution pattern of lumbar epaxial, especially M. multifidus motoneurons in the spinal cord of the cat: a study by the retrograde horseradish peroxidase method]. 1987

Y Kurosawa, and M Aoki

Distribution patterns of motoneurons supplying the lumbar epaxial muscles in the spinal ventral horn have been investigated by the retrograde horseradish peroxidase (HRP) method in 6 cats. HRP (30-40%, 3.0-5.7 mg) injections into multifidus muscle in 5 cases, and for comparison, longissimus lumborum muscle in one case were made at vertebral levels from L5 to L7 at multiple sites. After two days, the animals were sacrificed and frozen sections (60 microns thick) in the frontal plane of the spinal cord were made from the L1 to L7 segments and each section reacted histochemically with the tetramethylbenzidine (TMB) method. The results are summarized as follows. Following HRP injections into the multifidus muscle, labeled cells were found in the ventral horn rostrocaudally from the L2 to L6 segments. Total number of labeled cells ranged from 176 to 436 on the injected side. Labeled cells were most numerous in the L4 segment (range: 98-244). Labeled cells were found on the medial side of the ventral horn, especially in the ventromedial part (VM), predominantly ipsilateral to the injected muscle. Following HRP injection into the longissimus lumborum muscle in one case, labeled cells were also found on the medial side, but were more restricted in the centromedial part (CM) of the ventral horn. In all cases with HRP injection into the multifidus muscle, labeled cells were occasionally observed in VM and/or the medial ventral column (VC) contralateral to the injected muscle in L3 and/or L4 segment. The total number of these cells ranged from 2 to 66. These contralaterally labeled cells were presumed to be due to the peripheral HRP spread. Labeled cells observed in VM and CM were fusiform and round in shape, respectively. These cells had mean average soma diameters (ASD) from 32 to 40 microns and were presumed to be alpha motoneurons. A few labeled cells observed in VC were fusiform in shape and were significantly smaller (mean ASD: 23-28 microns) than the cells observed in other areas (VM, CM). In some cases, the dendrites of labeled cells in VM and VC extended dorsomedially in the VC across the midline in the anterior white commissure.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D009046	Motor Neurons	Neurons which activate MUSCLE CELLS.	Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D009132	Muscles	Contractile tissue that produces movement in animals.	Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D005260	Female		Females
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000870	Anterior Horn Cells	MOTOR NEURONS in the anterior (ventral) horn of the SPINAL CORD which project to SKELETAL MUSCLES.	Anterior Horn Neurons,Neurons, Anterior Horn,Neurons, Ventral Horn,Ventral Horn Cells,Ventral Horn Neurons,Anterior Horn Cell,Anterior Horn Neuron,Cell, Anterior Horn,Cell, Ventral Horn,Cells, Anterior Horn,Cells, Ventral Horn,Neuron, Anterior Horn,Neuron, Ventral Horn,Ventral Horn Cell,Ventral Horn Neuron
D001370	Axonal Transport	The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)	Axoplasmic Flow,Axoplasmic Transport,Axoplasmic Streaming,Axonal Transports,Axoplasmic Flows,Axoplasmic Transports,Streaming, Axoplasmic,Transport, Axonal,Transport, Axoplasmic,Transports, Axonal,Transports, Axoplasmic