Development and validation of a lateral flow assay for the detection of crustacean protein in processed foods. 2014

Daisuke Koizumi, and Kazuya Shirota, and Ryoko Akita, and Hiroshi Oda, and Hiroshi Akiyama
Central Research Institute, Maruha Nichiro Holdings, Inc., 16-2 Wadai, Tsukuba, Ibaraki 300-4295, Japan. Electronic address: d-koizumi@maruha-nichiro.co.jp.

We developed and validated a novel lateral flow assay for the detection of crustacean protein in processed foods. This assay had high sensitivity; the visual detection limit for shrimp protein extract was 25μg/L, equivalent to 1μg/g protein in a food sample, and results could be obtained within 20min without sophisticated procedures or expensive equipment. Concordance between our assay and another validated quantitative enzyme-linked immunosorbent assay was 97% for commercially processed foods. This assay is rapid, simple, reliable, and highly correlated with validated enzyme-linked immunosorbent assays and is thus suitable for monitoring of food products, especially in food-processing facilities.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D003445 Crustacea A large subphylum of mostly marine ARTHROPODS containing over 42,000 species. They include familiar arthropods such as lobsters (NEPHROPIDAE), crabs (BRACHYURA), shrimp (PENAEIDAE), and barnacles (THORACICA). Ostracoda,Ostracods,Crustaceas,Ostracod,Ostracodas
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D000485 Allergens Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE). Allergen
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D057140 Fast Foods Prepared food that is ready to eat or partially prepared food that has a final preparation time of a few minutes or less. Convenience Foods,Meals, Ready-To-Eat,Ready-Prepared Foods,Ready-To-Eat Meals,Convenience Food,Fast Food,Food, Convenience,Food, Fast,Food, Ready-Prepared,Foods, Convenience,Foods, Fast,Foods, Ready-Prepared,Meal, Ready-To-Eat,Meals, Ready To Eat,Ready Prepared Foods,Ready To Eat Meals,Ready-Prepared Food,Ready-To-Eat Meal
D057230 Limit of Detection Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure. Limits of Detection,Detection Limit,Detection Limits
D060829 Arthropod Proteins Proteins synthesized by organisms belonging to the phylum ARTHROPODA. Included in this heading are proteins from the subdivisions ARACHNIDA; CRUSTACEA; and HORSESHOE CRABS. Note that a separate heading for INSECT PROTEINS is listed under this heading. Arachnid Proteins,Crustacean Proteins,Horseshoe Crab Proteins,Spider Proteins,Tick Proteins

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