Biomaterial Database

Surface modification and bioconjugation of FeCo magnetic nanoparticles with proteins. 2014

Wei Wang, and Ying Jing, and Shihai He, and Jian-Ping Wang, and Jian-Ping Zhai

Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455, USA; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210046, PR China.

Magnetic Fe70Co30 nanoparticles with a cubic shape and a mean size of 15±1.5 nm were fabricated using a magnetron-sputtering-based gas phase condensation deposition method. The particles had a high saturation magnetization of 220 emu/g, which is much higher than that of commercially available iron oxide nanoparticles. The FeCo nanoparticles were modified by 3-aminopropyltriethoxy silane and subsequently activated by glutaraldehyde, leading to successful attachment of aldehyde groups onto nanoparticle surfaces. Three proteins, namely streptavidin, PAPP-A antibody and Nectin-4 antibody, were immobilized on glutaraldehyde activated FeCo nanoparticles, and their loading levels were quantitatively evaluated. Our results show that loading capabilities are 95 μg of streptavidin, 128 μg of PAPP-A, and 125 μg of Nectin-4 antibody per milligram of FeCo nanoparticles, and that the three immobilized proteins retain their binding bioactivity. The protein-FeCo conjugates may find valuable applications involving magnetic separation and purification of proteins and cells, and the magnetic detection of biomolecules.

UI	MeSH Term	Description	Entries
D007074	Immunoglobulin G	The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.	Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D007501	Iron	A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.	Iron-56,Iron 56
D008856	Microscopy, Fluorescence	Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.	Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D010316	Particle Size	Relating to the size of solids.	Particle Sizes,Size, Particle,Sizes, Particle
D003035	Cobalt	A trace element that is a component of vitamin B12. It has the atomic symbol Co, atomic number 27, and atomic weight 58.93. It is used in nuclear weapons, alloys, and pigments. Deficiency in animals leads to anemia; its excess in humans can lead to erythrocytosis.	Cobalt-59,Cobalt 59
D000906	Antibodies	Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D013499	Surface Properties	Characteristics or attributes of the outer boundaries of objects, including molecules.	Properties, Surface,Property, Surface,Surface Property
D056951	Photoelectron Spectroscopy	The study of the energy of electrons ejected from matter by the photoelectric effect, i.e., as a direct result of absorption of energy from electromagnetic radiation. As the energies of the electrons are characteristic of a specific element, the measurement of the energy of these electrons is a technique used to determine the chemical composition of surfaces.	Photoelectron Spectrometry,Electron Spectroscopy for Chemical Analysis,Photoelectron Emission Spectroscopy,Photoemission Spectroscopy,Ultra-Violet Photoelectron Spectroscopy,Ultraviolet Photoelectron Spectroscopy,X-Ray Photoelectron Spectroscopy,Xray Photoelectron Spectroscopy,Emission Spectroscopies, Photoelectron,Emission Spectroscopy, Photoelectron,Photoelectron Spectroscopy, Ultra-Violet,Photoelectron Spectroscopy, Ultraviolet,Photoelectron Spectroscopy, X-Ray,Photoelectron Spectroscopy, Xray,Spectrometry, Photoelectron,Spectroscopy, Photoelectron,Spectroscopy, Photoelectron Emission,Spectroscopy, Photoemission,Spectroscopy, Ultra-Violet Photoelectron,Spectroscopy, Ultraviolet Photoelectron,Spectroscopy, X-Ray Photoelectron,Spectroscopy, Xray Photoelectron,Ultra Violet Photoelectron Spectroscopy,X Ray Photoelectron Spectroscopy
D058185	Magnetite Nanoparticles	Synthesized magnetic particles under 100 nanometers used in many biomedical applications including DRUG DELIVERY SYSTEMS and CONTRAST AGENTS. The particles are usually coated with a variety of polymeric compounds.	Magnetite SPIONs,Magnetite Superparamagnetic Iron Oxide Nanoparticles,Superparamagnetic Magnetite Nanoparticles,Magnetite Nanoparticle,Magnetite Nanoparticle, Superparamagnetic,Magnetite Nanoparticles, Superparamagnetic,Magnetite SPION,Nanoparticle, Magnetite,Nanoparticle, Superparamagnetic Magnetite,Nanoparticles, Magnetite,Nanoparticles, Superparamagnetic Magnetite,SPION, Magnetite,SPIONs, Magnetite,Superparamagnetic Magnetite Nanoparticle
D060328	Magnetic Phenomena	Characteristics, properties, and effects of magnetic substances and magnetic fields.	Magnetic Phenomenon,Phenomena, Magnetic,Phenomenon, Magnetic