-transparent.png){kind=logo}
Synthesis of γ-labeled nucleoside 5'-triphosphates using click chemistry. 2014
Department of Chemistry, Ludwig-Maximilians Universität München, Butenandtstraße 5-13, 81377 Munich, Germany. thomas.carell@cup.uni-muenchen.de.
Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of γ-alkyne modified triphosphate amidates that are converted into a variety of γ-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.
Related Publications
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
January 1975,
Biofizika,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
May 2008,
Organic letters,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
November 2014,
Bioconjugate chemistry,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
June 2016,
Nucleosides, nucleotides & nucleic acids,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
December 1982,
Analytical biochemistry,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
July 2011,
Chemical communications (Cambridge, England),
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
October 1968,
FEBS letters,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
May 2007,
Carbohydrate research,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
December 2005,
Biochemical pharmacology,
S Serdjukow, and
F Kink, and
B Steigenberger, and
M Tomás-Gamasa, and
T Carell
December 1967,
Analytical biochemistry,
