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Endothelial vesicles on the luminal front of retinal and iridial vessels were studied by perfusion fixation after intravenous injection of peroxidase, and by perfusion fixation of glutaraldehyde with tannic acid or ruthenium red. Many luminal vesicles contained peroxidase reaction product after intravenous injection of peroxidase, but were virtually empty after perfusion fixation. Both tannic acid and ruthenium red delineated the majority of luminal vesicles, singular or in clusters, surface-associated or "free" in the cytoplasm. Only a few free vesicles were unlabeled by the dye. No transendothelial channels were seen. The results suggest that most of the vesicles on the luminal front of endothelial cells are continuous with the cell surface at the time of fixation. The function of these vesicles is not fully understood; some appear to be involved in endocytosis of macromolecules from the vascular lumen.
W L Lin, and
E Essner
December 1975,
Experimental eye research,
W L Lin, and
E Essner
October 1950,
The British journal of ophthalmology,
W L Lin, and
E Essner
October 1967,
Investigative ophthalmology,
W L Lin, and
E Essner
January 1979,
Canadian journal of ophthalmology. Journal canadien d'ophtalmologie,
W L Lin, and
E Essner
March 1987,
Scanning microscopy,
W L Lin, and
E Essner
May 1962,
The British journal of ophthalmology,
W L Lin, and
E Essner
August 2002,
Retina (Philadelphia, Pa.),
W L Lin, and
E Essner
September 1995,
Journal of neuroimmunology,
W L Lin, and
E Essner
January 2000,
Advances in experimental medicine and biology,
W L Lin, and
E Essner
November 1997,
The American journal of physiology,
