BIOMAT Database Logo BIOMAT Database Logo

The use of non-denaturing Deriphat-polyacrylamide gel electrophoresis to fractionate pigment-protein complexes of purple bacteria. 1991

L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Department of Botany, University of Glasgow, G12 8QQ, Glasgow, UK.

The suitability of Deriphat-polyacrylamide gel electrophoresis as a method for separating purple bacterial pigment-protein complexes has been tested. When appropriate non-denaturing detergents are used to solubilize chromatophores, this method provides a rapid, easy and microscale procedure for analyzing the composition of the bacterial photosynthetic apparatus with minimal disruption of individual pigment-proteins. Its usefulness is further illustrated by employing it to test for suitable detergents with which to solubilize purple bacterial chromatophores, and as an assay to study variation in the composition of the photosynthetic unit of bacterial cultures grown under different conditions.

UI MeSH Term Description Entries

Related Publications

L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Separation of chlorophyll-protein complexes by Deriphat polyacrylamide gradient gel electrophoresis.
January 1994, Electrophoresis,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
[Isolation of pigment-lipoprotein complexes from purple photosynthesizing bacteria by the method of preparative polyacrylamide gel electrophoresis].
January 1974, Mikrobiologiia,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Denaturing polyacrylamide gel electrophoresis.
May 2001, Current protocols in nucleic acid chemistry,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Denaturing polyacrylamide gel electrophoresis.
May 2001, Current protocols in human genetics,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis.
October 2016, Journal of visualized experiments : JoVE,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Chiroptical properties of chlorophyll-protein complexes separated on Deriphat/polyacrylamide gel.
January 1982, The Biochemical journal,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Non-denaturing polyacrylamide gradient gel electrophoresis for the diagnosis of dysbetalipoproteinemia.
January 2003, Journal of lipid research,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Denaturing urea polyacrylamide gel electrophoresis (Urea PAGE).
October 2009, Journal of visualized experiments : JoVE,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
2-D gel densitometer for high-contrast and selective imaging of chlorophyll-containing protein complexes separated by non-denaturing polyacrylamide gel electrophoresis.
May 2002, Journal of biochemical and biophysical methods,
L Ferguson, and E Halloran, and A M Hawthornthwaite, and R Cogdell, and C Kerfeld, and G F Peter, and J P Thornber
Purification of oligonucleotides using denaturing polyacrylamide gel electrophoresis.
May 2001, Current protocols in molecular biology,
Copied contents to your clipboard!