-transparent.png){kind=logo}
A series of 11 different monoclonal antibodies generated against human kidney renin have been characterised. Their binding affinity, inhibition of renin activity, epitope distribution, crossreactivity with related enzymes and finally in vivo pharmacological effects were analysed. All antibodies were found to be specific for primate renin recognising 6 independent antigenic structures on the renin molecule. They expressed different effects on renin activity namely (1) no inhibition, (2) only partial, or (3) complete inhibition. Partially inhibiting antibodies demonstrated specific degrees of inhibition (30, 60 or 80%). One antibody, R-36-16, demonstrated an IC 50 of 1.3 X 10(-11) M/L and, when injected into marmosets, induced complete inhibition of plasma renin activity and reduction of blood pressure. Using a selected pair of antibodies a radioimmunoassay has been established providing a fast and highly reproducible determination of human and marmoset immunoreactive renin, detecting both active and inactive renin down to concentrations of 10 pg/ml (1.25 X 10(-17) moles of renin per 50 microliter sample).
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
March 1988,
Cellular and molecular neurobiology,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
January 1984,
Transactions of the Association of American Physicians,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
October 1981,
The Biochemical journal,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
February 1984,
Archives of pathology & laboratory medicine,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
July 1989,
Journal of lipid research,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
January 1982,
Doklady Akademii nauk SSSR,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
December 1991,
The American journal of the medical sciences,
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
March 1988,
Hypertension (Dallas, Tex. : 1979),
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
January 1981,
Hypertension (Dallas, Tex. : 1979),
C H Heusser, and
J P Bews, and
S S Alkan, and
F M Dietrich, and
J M Wood, and
M de Gasparo, and
K G Hofbauer
January 1987,
Clinical and experimental hypertension. Part A, Theory and practice,
