Biomaterial Database

Survival of Mycobacterium avium subsp. paratuberculosis in ultra-filtered white cheese. 2014

S Hanifian

Department of Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

Mycobacterium avium subsp. paratuberculosis (Map) is considered as a potential public health threat due to its possible association with Crohn's disease in humans. This study aimed to investigate the survival of Map in ultra-filtered white (UFW) cheese, with special reference to the effect of initial inoculum level, addition of starter culture and salt concentration. Behaviour of Map was monitored during the storage of UFW cheese using F57-quantitative real-time PCR and culture assay. According to the results, higher inoculum level prolongs Map persistence in UFW cheese. Furthermore, the addition of starter culture was found as the main restrictive parameter affecting the survival of Map in UFW cheese. Meanwhile, salt concentration did not accelerate Map inactivation. It was concluded that UFW cheese has potential to support the survival of Map. CONCLUSIONS This study was conducted to investigate the factors that influence the survival of Mycobacterium avium subsp. paratuberculosis (Map) during the storage period of ultra-filtered white (UFW) cheese which represents a step forward to meet a critical industry need. Results revealed that the addition of starter culture could restrict the persistence of Map, while Map survival was not affected by higher salt concentration. In addition, persistence of Map was found dose-dependent. It was concluded that in UFW cheese, Map could survive the 60 days of storage period.

UI	MeSH Term	Description	Entries
D008892	Milk	The off-white liquid secreted by the mammary glands of humans and other mammals. It contains proteins, sugar, lipids, vitamins, and minerals.	Cow Milk,Cow's Milk,Milk, Cow,Milk, Cow's
D002611	Cheese	A nutritious food consisting primarily of the curd or the semisolid substance formed when milk coagulates.	Cheeses
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016927	Mycobacterium avium subsp. paratuberculosis	A subspecies of gram-positive, aerobic bacteria. It is the etiologic agent of Johne's disease (PARATUBERCULOSIS), a chronic GASTROENTERITIS in RUMINANTS.	Mycobacterium paratuberculosis,Mycobacterium avium paratuberculosis,Mycobacterium avium subspecies paratuberculosis
D060888	Real-Time Polymerase Chain Reaction	Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.	Kinetic Polymerase Chain Reaction,Quantitative Real-Time PCR,Quantitative Real-Time Polymerase Chain Reaction,Real-Time PCR,PCR, Quantitative Real-Time,PCR, Real-Time,PCRs, Quantitative Real-Time,PCRs, Real-Time,Quantitative Real Time PCR,Quantitative Real Time Polymerase Chain Reaction,Quantitative Real-Time PCRs,Real Time PCR,Real Time Polymerase Chain Reaction,Real-Time PCR, Quantitative,Real-Time PCRs,Real-Time PCRs, Quantitative
D061353	Food Storage	Keeping food for later consumption.	Shelf Life, Food,Food Shelf Life,Life, Food Shelf,Lifes, Food Shelf,Storage, Food