Relation between the expression of mitotic centromere-associated kinesin and the progression of squamous cell carcinoma of the tongue. 2014

Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
Lecturer, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.

OBJECTIVE Mitotic centromere-associated kinesin (MCAK) is a microtubule depolymerase indispensable for microtubule binding during spindle formation. The purpose of this study was to investigate the association of MCAK expression with squamous cell carcinoma of the oral tongue (SCCOT). METHODS Immunohistochemistry was used in 47 cases of SCCOT. Determination of proliferation and migratory capabilities was performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Transwell chamber assay, respectively, on cells from the human tongue squamous cell carcinoma cell line Tca8113 that were transfected with MCAK small interfering RNA (siRNA). RESULTS MCAK expression level in oral tongue cancer tissue is significantly higher (P < .01) than that of corresponding normal tissue. In addition, high expression of MCAK is significantly associated with lymph node metastasis (P < .05) and tumor staging (P < .01). Moreover, gene silencing of MCAK suppresses proliferation and migration of Tca8113 cells (P < .05; P < .01). CONCLUSIONS The expression of MCAK may be associated with the progression of SCCOT.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008207 Lymphatic Metastasis Transfer of a neoplasm from its primary site to lymph nodes or to distant parts of the body by way of the lymphatic system. Lymph Node Metastasis,Lymph Node Metastases,Lymphatic Metastases,Metastasis, Lymph Node
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009363 Neoplasm Proteins Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm. Proteins, Neoplasm
D009367 Neoplasm Staging Methods which attempt to express in replicable terms the extent of the neoplasm in the patient. Cancer Staging,Staging, Neoplasm,Tumor Staging,TNM Classification,TNM Staging,TNM Staging System,Classification, TNM,Classifications, TNM,Staging System, TNM,Staging Systems, TNM,Staging, Cancer,Staging, TNM,Staging, Tumor,System, TNM Staging,Systems, TNM Staging,TNM Classifications,TNM Staging Systems
D002294 Carcinoma, Squamous Cell A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed) Carcinoma, Epidermoid,Carcinoma, Planocellular,Carcinoma, Squamous,Squamous Cell Carcinoma,Carcinomas, Epidermoid,Carcinomas, Planocellular,Carcinomas, Squamous,Carcinomas, Squamous Cell,Epidermoid Carcinoma,Epidermoid Carcinomas,Planocellular Carcinoma,Planocellular Carcinomas,Squamous Carcinoma,Squamous Carcinomas,Squamous Cell Carcinomas
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
December 1997, Biochimica et biophysica acta,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
October 2011, The Journal of biological chemistry,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
August 1998, The Journal of cell biology,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
December 2011, Oncotarget,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
January 2011, The Journal of biological chemistry,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
January 2008, Cancer science,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
September 2001, The Journal of biological chemistry,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
May 2019, Biochemical and biophysical research communications,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
March 2015, Oncotarget,
Cheng-Qin Wang, and Feng-Gang Xiang, and Yu-Jun Li, and Xiao-Ming Xing, and Ning Wang, and Jing-Hua Chi, and Wen-Juan Yu
July 2009, Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology,
Copied contents to your clipboard!