BIOMAT Database Logo BIOMAT Database Logo

Sulfation of polysaccharides generates potent and selective inhibitors of human immunodeficiency virus infection and replication in vitro. 1987

H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Ube.

The inhibitory effects of several polysaccharides, dextran, xylofuranan, and ribofuranan, and their sulfated counterparts on the infectivity and replication of human immunodeficiency virus (HIV) were examined by using an HTLV-I-carrying cell line, MT-4, in vitro. Dextran sulfate (Mw 34 X 10(3], xylofuranan sulfate, and ribofuranan sulfate completely prevented HIV-induced cytopathic effects (CPE) at concentrations greater than 10 micrograms/ml and dextran sulfate (Mw 7 X 10(3] at concentrations greater than 100 micrograms/ml. However, the non-sulfated compounds did not prevent them at any concentration tested. The anti-HIV effect of these polysaccharides was confirmed by measuring HIV-specific antigen expression in infected MT-4 cells. In cocultures with MOLT-4 and MOLT-4/HIVHTLV-IIIB cells, formation of multinucleated cells was completely inhibited in the presence of 100 micrograms/ml of these sulfated compounds. Dextran sulfate showed 20-30% growth inhibition of uninfected MT-4 cells at 1000 micrograms/ml but dextran sulfate, xylofuranan sulfate, and ribofuranan sulfate showed no effect on sulfated polysaccharides efficiently inhibited the reverse transcriptase activity of avian myeloblastosis virus and HIV.

UI MeSH Term Description Entries
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D006678 HIV Human immunodeficiency virus. A non-taxonomic and historical term referring to any of two species, specifically HIV-1 and/or HIV-2. Prior to 1986, this was called human T-lymphotropic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV). From 1986-1990, it was an official species called HIV. Since 1991, HIV was no longer considered an official species name; the two species were designated HIV-1 and HIV-2. AIDS Virus,HTLV-III,Human Immunodeficiency Viruses,Human T-Cell Lymphotropic Virus Type III,Human T-Lymphotropic Virus Type III,LAV-HTLV-III,Lymphadenopathy-Associated Virus,Acquired Immune Deficiency Syndrome Virus,Acquired Immunodeficiency Syndrome Virus,Human Immunodeficiency Virus,Human T Cell Lymphotropic Virus Type III,Human T Lymphotropic Virus Type III,Human T-Cell Leukemia Virus Type III,Immunodeficiency Virus, Human,Immunodeficiency Viruses, Human,Virus, Human Immunodeficiency,Viruses, Human Immunodeficiency,AIDS Viruses,Human T Cell Leukemia Virus Type III,Lymphadenopathy Associated Virus,Lymphadenopathy-Associated Viruses,Virus, AIDS,Virus, Lymphadenopathy-Associated,Viruses, AIDS,Viruses, Lymphadenopathy-Associated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000998 Antiviral Agents Agents used in the prophylaxis or therapy of VIRUS DISEASES. Some of the ways they may act include preventing viral replication by inhibiting viral DNA polymerase; binding to specific cell-surface receptors and inhibiting viral penetration or uncoating; inhibiting viral protein synthesis; or blocking late stages of virus assembly. Antiviral,Antiviral Agent,Antiviral Drug,Antivirals,Antiviral Drugs,Agent, Antiviral,Agents, Antiviral,Drug, Antiviral,Drugs, Antiviral
D013431 Sulfates Inorganic salts of sulfuric acid. Sulfate,Sulfates, Inorganic,Inorganic Sulfates
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D018894 Reverse Transcriptase Inhibitors Inhibitors of reverse transcriptase (RNA-DIRECTED DNA POLYMERASE), an enzyme that synthesizes DNA on an RNA template. Reverse Transcriptase Inhibitor,Inhibitors, Reverse Transcriptase,Inhibitor, Reverse Transcriptase,Transcriptase Inhibitor, Reverse

Related Publications

H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Novel sulfated polymers as highly potent and selective inhibitors of human immunodeficiency virus replication and giant cell formation.
January 1990, Antimicrobial agents and chemotherapy,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
The molecular target of bicyclams, potent inhibitors of human immunodeficiency virus replication.
February 1996, Journal of virology,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus, and human immunodeficiency virus.
November 1988, Antimicrobial agents and chemotherapy,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Thiadiazole derivatives: highly potent and selective inhibitors of human immunodeficiency virus type 1 (HIV-1) replications in vitro.
January 1997, Microbiology and immunology,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Indolocarbazoles: potent, selective inhibitors of human cytomegalovirus replication.
June 1999, Bioorganic & medicinal chemistry,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Both 2',3'-dideoxythymidine and its 2',3'-unsaturated derivative (2',3'-dideoxythymidinene) are potent and selective inhibitors of human immunodeficiency virus replication in vitro.
January 1987, Biochemical and biophysical research communications,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
APOBEC3B and APOBEC3C are potent inhibitors of simian immunodeficiency virus replication.
December 2004, The Journal of biological chemistry,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Potent and selective inhibitors of human immunodeficiency virus protease structurally related to L-694,746.
July 1998, Antiviral chemistry & chemotherapy,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Ruxolitinib and tofacitinib are potent and selective inhibitors of HIV-1 replication and virus reactivation in vitro.
January 2014, Antimicrobial agents and chemotherapy,
H Nakashima, and O Yoshida, and T S Tochikura, and T Yoshida, and T Mimura, and Y Kido, and Y Motoki, and Y Kaneko, and T Uryu, and N Yamamoto
Selective activity of several cholic acid derivatives against human immunodeficiency virus replication in vitro.
January 1989, Journal of acquired immune deficiency syndromes,
Copied contents to your clipboard!