BIOMAT Database Logo BIOMAT Database Logo

Anti-human tumor antibodies induced in mice and rabbits by "internal image" anti-idiotypic monoclonal immunoglobulins. 1987

G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Dipartimento di Biologia e Genetica per le Scienze Mediche, Universitã degli Studi, Milano, Italy.

MBr1 is a murine monoclonal antibody, defining a saccharidic epitope [CaMBr1] of a human tissue-specific, tumor-associated globoside, present on the mammary carcinoma cell line MCF-7. The same epitope is shared by glycoproteins present on normal and neoplastic mammary epithelial cells, and by mucins from some ovarian cyst fluids. We have used MBr1 as the monoclonal antitumor antibody in an idiotypic sequence of immunizations in order to obtain and characterize "internal images" of the original epitope to be used as substitutes of the nominal antigen in serologic immunoassays. Two monoclonal anti-idiotypic antibodies (beta-1 and beta-2), which reacted with paratope-related idiotopes on MBr1, were obtained. The analysis of the antigenic and immunogenic properties of these molecules by both "antigen" and "antibody" competition assays provided evidence that both beta-1 and beta-2 bear "internal images" of the MBr1-defined epitope. Moreover, when injected in mice and rabbits both beta-1 and beta-2 induced anti anti-idiotypic antibodies, which mimicked MBr1 in binding MCF-7 as well as normal and neoplastic mammary gland epithelial cells. These data are discussed in terms of their possible application to the production of tumor-associated antigen substitutes and their use in serologic immunoassays.

UI MeSH Term Description Entries
D007130 Immunoglobulin Idiotypes Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains. Idiotypes, Immunoglobulin,Ig Idiotypes,Idiotype, Ig,Idiotype, Immunoglobulin,Idiotypes, Ig,Ig Idiotype,Immunoglobulin Idiotype
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D011817 Rabbits A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes. Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D001943 Breast Neoplasms Tumors or cancer of the human BREAST. Breast Cancer,Breast Tumors,Cancer of Breast,Breast Carcinoma,Cancer of the Breast,Human Mammary Carcinoma,Malignant Neoplasm of Breast,Malignant Tumor of Breast,Mammary Cancer,Mammary Carcinoma, Human,Mammary Neoplasm, Human,Mammary Neoplasms, Human,Neoplasms, Breast,Tumors, Breast,Breast Carcinomas,Breast Malignant Neoplasm,Breast Malignant Neoplasms,Breast Malignant Tumor,Breast Malignant Tumors,Breast Neoplasm,Breast Tumor,Cancer, Breast,Cancer, Mammary,Cancers, Mammary,Carcinoma, Breast,Carcinoma, Human Mammary,Carcinomas, Breast,Carcinomas, Human Mammary,Human Mammary Carcinomas,Human Mammary Neoplasm,Human Mammary Neoplasms,Mammary Cancers,Mammary Carcinomas, Human,Neoplasm, Breast,Neoplasm, Human Mammary,Neoplasms, Human Mammary,Tumor, Breast
D005915 Globosides Glycosphingolipids containing N-acetylglucosamine (paragloboside) or N-acetylgalactosamine (globoside). Globoside is the P antigen on erythrocytes and paragloboside is an intermediate in the biosynthesis of erythrocyte blood group ABH and P 1 glycosphingolipid antigens. The accumulation of globoside in tissue, due to a defect in hexosaminidases A and B, is the cause of Sandhoff disease. Cytolipins,Lacto-N-neotetraosylceramide,Lacto-N-tetraosylceramide,Lactoneotetraosylceramide,Lacto N neotetraosylceramide,Lacto N tetraosylceramide
D006023 Glycoproteins Conjugated protein-carbohydrate compounds including MUCINS; mucoid, and AMYLOID glycoproteins. C-Glycosylated Proteins,Glycosylated Protein,Glycosylated Proteins,N-Glycosylated Proteins,O-Glycosylated Proteins,Glycoprotein,Neoglycoproteins,Protein, Glycosylated,Proteins, C-Glycosylated,Proteins, Glycosylated,Proteins, N-Glycosylated,Proteins, O-Glycosylated
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000912 Antibodies, Neoplasm Immunoglobulins induced by antigens specific for tumors other than the normally occurring HISTOCOMPATIBILITY ANTIGENS. Neoplasm Antibodies,Tumor Antibodies,Antibodies, Tumor

Related Publications

G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Internal image-bearing anti-idiotypic monoclonal antibodies.
January 1995, Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Human CD4 "internal antigen" mimicry by anti-idiotypic monoclonal antibodies.
January 1994, International journal of clinical & laboratory research,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Analysis of anti-tumor antibodies in mice and rabbits induced by monoclonal anti-idiotope antibodies.
September 1991, Journal of immunology (Baltimore, Md. : 1950),
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Anti-idiotypic antibodies bear the internal image of a human tumor antigen.
April 1986, Science (New York, N.Y.),
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Monoclonal 'internal image' anti-idiotypic antibodies of hepatitis B surface antigen.
June 1985, Immunology,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
The common occurrence of internal image type anti-idiotypic antibodies in rabbits immunized with monoclonal and polyclonal human IgM rheumatoid factors.
June 1986, Clinical and experimental immunology,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
A conserved coronavirus epitope, critical in virus neutralization, mimicked by internal-image monoclonal anti-idiotypic antibodies.
December 1991, Journal of virology,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Human monoclonal anti-idiotypic antibodies bearing the internal image of hemorrhagic fever with renal syndrome virus antigen.
January 1994, Human antibodies and hybridomas,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Generation of internal image monoclonal anti-idiotypic antibodies against idiotypic antibodies to GP5 antigen of porcine reproductive and respiratory syndrome virus.
May 2008, Journal of virological methods,
G Viale, and F Grassi, and M Pelagi, and R Alzani, and S Ménard, and S Miotti, and R Buffa, and A Gini, and A G Siccardi
Anti-tumor effect of internal image bearing anti-idiotypic monoclonal antibody in relation to GM3 ganglioside.
May 1998, International journal of cancer,
Copied contents to your clipboard!