BIOMAT Database Logo BIOMAT Database Logo

Purification and partial characterization of fimbriae of Vibrio cholerae O1. 1987

M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Department of Bacteriology, Nagasaki University, Japan.

Fimbriae of Vibrio cholerae O1 were purified from a strain of the classical biotype, Inaba serotype (Bgd 17), and a strain of the El Tor biotype, Ogawa serotype (K23), grown on TCG agar medium by the following procedure; homogenization of the cell suspension to detach fimbriae, ultracentrifugation to remove remaining cells and their debris, concentration of the supernatant containing fimbriae with ultrafiltration, and 20 to 40% sucrose linear gradient centrifugation of the concentrated material. The fimbriae in both preparations were flexible, long fibres readily distinguishable under the electron microscope from those of CFA/I, CFA/II seen in ETEC strains. Their structural subunit was a protein of 16 kdaltons. Fimbriae isolated from both serotypes and biotypes shared antigenic determinants.

UI MeSH Term Description Entries
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D009439 Neuraminidase An enzyme that catalyzes the hydrolysis of alpha-2,3, alpha-2,6-, and alpha-2,8-glycosidic linkages (at a decreasing rate, respectively) of terminal sialic residues in oligosaccharides, glycoproteins, glycolipids, colominic acid, and synthetic substrate. (From Enzyme Nomenclature, 1992) Sialidase,Exo-alpha-Sialidase,N-Acylneuraminate Glycohydrolases,Oligosaccharide Sialidase,Exo alpha Sialidase,Glycohydrolases, N-Acylneuraminate,N Acylneuraminate Glycohydrolases,Sialidase, Oligosaccharide
D010861 Fimbriae, Bacterial Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX). Bacterial Fimbriae,Bacterial Pili,Common Fimbriae,Common Pili,Pili, Bacterial,Pili, Common,Bacterial Fimbria,Bacterial Pilus,Common Fimbria,Common Pilus,Fimbria, Bacterial,Pilus, Bacterial,Fimbria, Common,Fimbriae, Common,Pilus, Common
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006384 Hemagglutination The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL). Hemagglutinations
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000939 Epitopes Sites on an antigen that interact with specific antibodies. Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D000942 Antigens, Bacterial Substances elaborated by bacteria that have antigenic activity. Bacterial Antigen,Bacterial Antigens,Antigen, Bacterial
D014734 Vibrio cholerae The etiologic agent of CHOLERA. Bacillus cholerae,Bacillus cholerae-asiaticae,Liquidivibrio cholerae,Microspira comma,Pacinia cholerae-asiaticae,Spirillum cholerae,Spirillum cholerae-asiaticae,Vibrio albensis,Vibrio cholera,Vibrio cholerae-asiaticae,Vibrio comma

Related Publications

M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Purification and characterization of vibrio cholerae O139 fimbriae.
January 1994, FEMS microbiology letters,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Non-O1 Vibrio cholerae hemolysin: purification, partial characterization, and immunological relatedness to El Tor hemolysin.
July 1984, Infection and immunity,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin.
April 1986, Infection and immunity,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Purification and characterization of novel toxin produced by Vibrio cholerae O1.
October 1999, Infection and immunity,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Two-step purification and partial characterization of a variant of the Vibrio cholerae non-O1 hemolysin.
November 1999, FEMS microbiology letters,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Production and partial characterization of pili on non-O1 Vibrio cholerae.
January 1988, The Journal of infectious diseases,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Immunogenicity of Vibrio cholerae O1 fimbriae in animal and human cholera.
January 1993, Microbiology and immunology,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Purification and characterization of pili of a Vibrio cholerae non-O1 strain.
December 1993, Infection and immunity,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Production and partial purification of a fluid-accumulating factor of non-O1 Vibrio cholerae.
January 1988, Microbiology and immunology,
M Ehara, and M Ishibashi, and Y Ichinose, and M Iwanaga, and S Shimotori, and T Naito
Purification and partial characterization of a non-O1 Vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of Vibrio parahaemolyticus.
April 1986, Infection and immunity,
Copied contents to your clipboard!