Biomaterial Database

Biochemical characterization and tissue distribution of the Cora antigen, a cell surface glycoprotein differentially expressed on malignant and benign gastrointestinal epithelia. 1988

H G Göttlinger, and F M Lobo, and T W Grimm, and G Riethmüller, and J P Johnson

Institute for Immunology, University of Munich, Federal Republic of Germany.

A monoclonal antibody designated Cora was isolated which discriminates between malignant and benign colon epithelium. It identifies a novel, variably glycosylated membrane glycoprotein. Expression of the Cora antigen was shown to be characteristic for gastrointestinal carcinomas (100% of tested colorectal carcinomas, 70% of tested gastric carcinomas) but could not be detected on normal gastrointestinal tissues using histochemical methods on frozen tissue sections. An extensive survey of normal tissues revealed that the Cora antigen has a very restricted distribution pattern, being detected only on alveoli of the lung, granulocytes, and bone marrow. Polyacrylamide gel electrophoresis of immunoprecipitates prepared from [35S]methionine, 125I, or [3H]glucosamine labeled colon carcinoma cell lines showed that the Cora antigen consists of a group of glycoproteins ranging in apparent molecular weight from 75,000 to 95,000. Following treatment with neuraminidase, the apparent molecular weights were reduced (65,000 to 85,000) but the size heterogeneity remained. Culturing the cells in the presence of tunicamycin, which inhibits N-linked glycosylation, removed this heterogeneity and under these conditions monoclonal antibody Cora precipitated a major band with an apparent molecular weight of 33,000. Because this monoclonal antibody can distinguish between normal and malignant gastrointestinal epithelia, expression of the Cora antigen may be associated with the process of malignant transformation in this tissue.

UI	MeSH Term	Description	Entries
D008814	Mice, Inbred NZB	An inbred strain of mouse that is widely used as a model for AUTOIMMUNE DISEASES such as SYSTEMIC LUPUS ERYTHEMATOSUS.	Mice, NZB,Mouse, Inbred NZB,Mouse, NZB,Inbred NZB Mice,Inbred NZB Mouse,NZB Mice,NZB Mice, Inbred,NZB Mouse,NZB Mouse, Inbred
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D003110	Colonic Neoplasms	Tumors or cancer of the COLON.	Cancer of Colon,Colon Adenocarcinoma,Colon Cancer,Cancer of the Colon,Colon Neoplasms,Colonic Cancer,Neoplasms, Colonic,Adenocarcinoma, Colon,Adenocarcinomas, Colon,Cancer, Colon,Cancer, Colonic,Cancers, Colon,Cancers, Colonic,Colon Adenocarcinomas,Colon Cancers,Colon Neoplasm,Colonic Cancers,Colonic Neoplasm,Neoplasm, Colon,Neoplasm, Colonic,Neoplasms, Colon
D004064	Digestive System	A group of organs stretching from the MOUTH to the ANUS, serving to breakdown foods, assimilate nutrients, and eliminate waste. In humans, the digestive system includes the GASTROINTESTINAL TRACT and the accessory glands (LIVER; BILIARY TRACT; PANCREAS).	Ailmentary System,Alimentary System
D004848	Epithelium	The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body.	Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005260	Female		Females
D005770	Gastrointestinal Neoplasms	Tumors or cancer of the GASTROINTESTINAL TRACT, from the MOUTH to the ANAL CANAL.	Gastrointestinal Cancer,Cancer of Gastrointestinal Tract,Cancer of the Gastrointestinal Tract,Neoplasms, Gastrointestinal,Cancer, Gastrointestinal,Cancers, Gastrointestinal,Gastrointestinal Cancers,Gastrointestinal Neoplasm,Gastrointestinal Tract Cancer,Gastrointestinal Tract Cancers,Neoplasm, Gastrointestinal
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic