Mechanism of Saccharomyces cerevisiae yeast cell death induced by heat shock. Effect of cycloheximide on thermotolerance. 2014

E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
Siberian Institute of Plant Physiology and Biochemistry, Siberian Division of the Russian Academy of Sciences, Irkutsk, 664033, Russia. fedoseeva@sifibr.irk.ru.

The mechanism of yeast cell death induced by heat shock was found to be dependent on the intensity of heat exposure. Moderate (45°C) heat shock strongly increased the generation of reactive oxygen species (ROS) and cell death. Pretreatment with cycloheximide (at 30°C) suppressed cell death, but produced no effect on ROS production. The protective effect was absent if cycloheximide was added immediately before heat exposure and the cells were incubated with the drug during the heat treatment and recovery period. The rate of ROS production and protective effect of cycloheximide on viability were significantly decreased in the case of severe (50°C) heat shock. Treatment with cycloheximide at 39°C inhibited the induction of Hsp104 synthesis and suppressed the development of induced thermotolerance to severe shock (50°C), but it had no effect on induced thermotolerance to moderate (45°C) heat shock. At the same time, Hsp104 effectively protected cells from death independently of the intensity of heat exposure. These data indicate that moderate heat shock induced programmed cell death in the yeast cells, and cycloheximide suppressed this process by inhibiting general synthesis of proteins.

UI MeSH Term Description Entries
D011500 Protein Synthesis Inhibitors Compounds which inhibit the synthesis of proteins. They are usually ANTI-BACTERIAL AGENTS or toxins. Mechanism of the action of inhibition includes the interruption of peptide-chain elongation, the blocking the A site of ribosomes, the misreading of the genetic code or the prevention of the attachment of oligosaccharide side chains to glycoproteins. Protein Synthesis Antagonist,Protein Synthesis Antagonists,Protein Synthesis Inhibitor,Antagonist, Protein Synthesis,Antagonists, Protein Synthesis,Inhibitor, Protein Synthesis,Inhibitors, Protein Synthesis,Synthesis Antagonist, Protein,Synthesis Inhibitor, Protein
D003513 Cycloheximide Antibiotic substance isolated from streptomycin-producing strains of Streptomyces griseus. It acts by inhibiting elongation during protein synthesis. Actidione,Cicloheximide
D006360 Heat-Shock Proteins Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions. Stress Protein,Stress Proteins,Heat-Shock Protein,Heat Shock Protein,Heat Shock Proteins,Protein, Stress
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D017382 Reactive Oxygen Species Molecules or ions formed by the incomplete one-electron reduction of oxygen. These reactive oxygen intermediates include SINGLET OXYGEN; SUPEROXIDES; PEROXIDES; HYDROXYL RADICAL; and HYPOCHLOROUS ACID. They contribute to the microbicidal activity of PHAGOCYTES, regulation of SIGNAL TRANSDUCTION and GENE EXPRESSION, and the oxidative damage to NUCLEIC ACIDS; PROTEINS; and LIPIDS. Active Oxygen Species,Oxygen Radical,Oxygen Radicals,Pro-Oxidant,Reactive Oxygen Intermediates,Active Oxygen,Oxygen Species, Reactive,Pro-Oxidants,Oxygen, Active,Pro Oxidant,Pro Oxidants,Radical, Oxygen
D029701 Saccharomyces cerevisiae Proteins Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. Baker's Yeast Proteins,S cerevisiae Proteins

Related Publications

E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
November 2000, The Biochemical journal,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
October 1982, Biochemical and biophysical research communications,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
December 1987, Radiation research,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
February 2020, Biochemical and biophysical research communications,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
January 2001, Mikrobiologiia,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
February 1987, Journal of bacteriology,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
December 1983, Genetika,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
July 2018, Pesticide biochemistry and physiology,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
August 1990, Journal of bacteriology,
E G Rikhvanov, and I V Fedoseeva, and N N Varakina, and T M Rusaleva, and A V Fedyaeva
March 1996, Biochemical and biophysical research communications,
Copied contents to your clipboard!