An inhibitory role for caspase-3 at the late stage of RANKL-induced osteoclast differentiation in RAW264 cells and mouse bone marrow macrophages. 2014

Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
Department of Orthodontics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Japan.

Osteoclast differentiation/activation is involved in orthodontic tooth movement at the compression sites of the alveolar bone. RANKL, a member of the TNF family expressed in osteoblasts, binds to RANK, a member of the TNF receptor family expressed on preosteoclasts, resulting in differentiation of preosteoclasts into mature osteoclasts. Several members of the TNF family, such as TNF and Fas ligand, can induce apoptosis by activation of caspase-3. We have investigated whether caspase-3 be involved in the late stage of RANKL-induced osteoclast differentiation. Increased active caspase-3 was found in mouse monocytic RAW264 cells differentiated into mature osteoclasts by treatment with RANKL for 3 days. Co-treatment with Z-Asp-CH₂-DCB, a caspase-3-specific inhibitor, augmented RANKL-induced osteoclast differentiation in RAW264 cells, also seen in mouse bone marrow macrophages. This suggests that activation of caspase-3 may play an inhibitory role at the late stage of RANKL-induced osteoclast differentiation.

UI MeSH Term Description Entries
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D009842 Oligopeptides Peptides composed of between two and twelve amino acids. Oligopeptide
D010010 Osteoclasts A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption. Odontoclasts,Cementoclast,Cementoclasts,Odontoclast,Osteoclast
D001854 Bone Marrow Cells Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells. Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D000539 Alveolar Process The thickest and spongiest part of the maxilla and mandible hollowed out into deep cavities for the teeth. Alveolar Ridge,Alveolar Processes,Process, Alveolar,Processes, Alveolar,Ridge, Alveolar
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001224 Aspartic Acid One of the non-essential amino acids commonly occurring in the L-form. It is found in animals and plants, especially in sugar cane and sugar beets. It may be a neurotransmitter. (+-)-Aspartic Acid,(R,S)-Aspartic Acid,Ammonium Aspartate,Aspartate,Aspartate Magnesium Hydrochloride,Aspartic Acid, Ammonium Salt,Aspartic Acid, Calcium Salt,Aspartic Acid, Dipotassium Salt,Aspartic Acid, Disodium Salt,Aspartic Acid, Hydrobromide,Aspartic Acid, Hydrochloride,Aspartic Acid, Magnesium (1:1) Salt, Hydrochloride, Trihydrate,Aspartic Acid, Magnesium (2:1) Salt,Aspartic Acid, Magnesium-Potassium (2:1:2) Salt,Aspartic Acid, Monopotassium Salt,Aspartic Acid, Monosodium Salt,Aspartic Acid, Potassium Salt,Aspartic Acid, Sodium Salt,Calcium Aspartate,Dipotassium Aspartate,Disodium Aspartate,L-Aspartate,L-Aspartic Acid,Magnesiocard,Magnesium Aspartate,Mg-5-Longoral,Monopotassium Aspartate,Monosodium Aspartate,Potassium Aspartate,Sodium Aspartate,Aspartate, Ammonium,Aspartate, Calcium,Aspartate, Dipotassium,Aspartate, Disodium,Aspartate, Magnesium,Aspartate, Monopotassium,Aspartate, Monosodium,Aspartate, Potassium,Aspartate, Sodium,L Aspartate,L Aspartic Acid

Related Publications

Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
January 2020, Biological & pharmaceutical bulletin,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
January 2020, Frontiers in pharmacology,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
January 2015, Biological & pharmaceutical bulletin,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
January 2011, Journal of pharmacological sciences,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
January 2024, Biochemical and biophysical research communications,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
March 2017, BMC complementary and alternative medicine,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
October 2015, Food & function,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
December 2017, The Journal of veterinary medical science,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
July 2020, Journal of ethnopharmacology,
Yuko Katao, and Mika Shishido, and Kaoru Inami, and Naoyuki Matsumoto, and Hirofumi Sawai
February 2011, Biomedical research (Tokyo, Japan),
Copied contents to your clipboard!