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A micromethod for the detection of human immunodeficiency virus (HIV) and other retroviruses in cell culture supernatants is described which applies a DEAE ion exchanger for recovery of polynucleotides synthesized in vitro by the retroviral reverse transcriptase. Cell culture, sample preparation, and test performance including the washing step are adapted to microtitre plates. Compared to the standard method this technique produced less non-specific reactions, resulting in a more than 3-fold higher sensitivity, a higher reproducibility due to lower intrarun variations and allowed an increase in the daily sample accomplishment per person 3- to 4-fold at lower costs per sample.
J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
March 1990,
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J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
March 2009,
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J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
June 1987,
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J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
June 1988,
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J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
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Biological chemistry Hoppe-Seyler,
J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
January 1988,
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J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
January 2013,
Bing du xue bao = Chinese journal of virology,
J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
July 2002,
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J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
January 1995,
Microbiology and immunology,
J P Gregersen, and
H Wege, and
L Preiss, and
K D Jentsch
September 1994,
The Journal of biological chemistry,
