A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. 2014

Céline Sorin, and Marie Declerck, and Aurélie Christ, and Thomas Blein, and Linnan Ma, and Christine Lelandais-Brière, and Maria Fransiska Njo, and Tom Beeckman, and Martin Crespi, and Caroline Hartmann
Institut des Sciences du Végétal (ISV), CNRS, UPR2355, Saclay Plant Sciences, F-91198, Gif-sur-Yvette Cedex, France.

In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.

UI MeSH Term Description Entries
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D017360 Arabidopsis A plant genus of the family BRASSICACEAE that contains ARABIDOPSIS PROTEINS and MADS DOMAIN PROTEINS. The species A. thaliana is used for experiments in classical plant genetics as well as molecular genetic studies in plant physiology, biochemistry, and development. Arabidopsis thaliana,Cress, Mouse-ear,A. thaliana,A. thalianas,Arabidopses,Arabidopsis thalianas,Cress, Mouse ear,Cresses, Mouse-ear,Mouse-ear Cress,Mouse-ear Cresses,thaliana, A.,thaliana, Arabidopsis,thalianas, A.
D059368 RNA Isoforms The different gene transcripts generated from a single gene by RNA EDITING or ALTERNATIVE SPLICING of RNA PRECURSORS. mRNA Isoform,Messenger RNA Isoforms,mRNA Isoforms,Isoform, mRNA,Isoforms, Messenger RNA,Isoforms, RNA,Isoforms, mRNA,RNA Isoforms, Messenger
D017930 Genes, Reporter Genes whose expression is easily detectable and therefore used to study promoter activity at many positions in a target genome. In recombinant DNA technology, these genes may be attached to a promoter region of interest. Reporter Genes,Gene, Reporter,Reporter Gene
D018506 Gene Expression Regulation, Plant Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in plants. Plant Gene Expression Regulation,Regulation of Gene Expression, Plant,Regulation, Gene Expression, Plant
D018517 Plant Roots The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Plant Bulbs,Plant Root,Bulb, Plant,Bulbs, Plant,Plant Bulb,Root, Plant,Roots, Plant
D018519 Meristem A group of plant cells that are capable of dividing infinitely and whose main function is the production of new growth at the growing tip of a root or stem. (From Concise Dictionary of Biology, 1990) Root Tip,Meristems,Root Tips,Tip, Root,Tips, Root
D018749 RNA, Plant Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis. Plant RNA
D023081 CCAAT-Binding Factor A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C. CCAAT-Binding Factor, A-Subunit,CCAAT-Binding Factor, B-Subunit,CCAAT-Binding Factor, C-Subunit,CBF Protein, CCAAT-Binding,CBF-A Protein, CCAAT-Binding,CBF-B Protein, CCAAT-Binding,CBF-C Protein, CCAAT-Binding,CCAAT-Binding Protein, CP1,CCAAT-Binding Transcription Factor,CCAAT-Binding-Factor,NF-Y Protein,CBF A Protein, CCAAT Binding,CBF B Protein, CCAAT Binding,CBF C Protein, CCAAT Binding,CBF Protein, CCAAT Binding,CCAAT Binding Factor,CCAAT Binding Factor, A Subunit,CCAAT Binding Factor, B Subunit,CCAAT Binding Factor, C Subunit,CCAAT Binding Protein, CP1,CCAAT Binding Transcription Factor,CCAAT-Binding CBF Protein,CCAAT-Binding CBF-A Protein,CCAAT-Binding CBF-B Protein,CCAAT-Binding CBF-C Protein,CP1 CCAAT-Binding Protein,Factor, A-Subunit CCAAT-Binding,Factor, B-Subunit CCAAT-Binding,Factor, C-Subunit CCAAT-Binding,NF Y Protein,Protein, CCAAT-Binding CBF-A,Protein, CCAAT-Binding CBF-B,Protein, NF-Y,Transcription Factor, CCAAT-Binding

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