An effective method for extracting total RNA from Dioscorea opposita Thunb. 2014

C M Liao, and J Li, and X H Liu, and Y S Zhang
Library, China West Normal University, Nanchong City, China.

Dioscorea opposita Thunb., included in the genus Dioscorea of the family Dioscoreaceae, is an important herb with great edible and medicinal value. In this study, the total RNA from leaves of Lichuan Dioscorea opposita Thunb. was isolated by an improved Trizol method. The results showed that the RNA extracted by the improved Trizol method had good integrity, and the RNA could be used for down-stream molecular biology operations including reverse transcription-polymerase chain reaction.

UI MeSH Term Description Entries
D005591 Chemical Fractionation Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Fractionation, Chemical,Chemical Fractionations,Fractionations, Chemical
D012313 RNA A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed) RNA, Non-Polyadenylated,Ribonucleic Acid,Gene Products, RNA,Non-Polyadenylated RNA,Acid, Ribonucleic,Non Polyadenylated RNA,RNA Gene Products,RNA, Non Polyadenylated
D018749 RNA, Plant Ribonucleic acid in plants having regulatory and catalytic roles as well as involvement in protein synthesis. Plant RNA
D027742 Dioscorea A plant genus best known for edible underground tubers. Yam may also refer to a moist variety of sweet potato, IPOMOEA BATATAS. Yam,Chinese Yam,Dioscorea batatas,Dioscorea opposita,Dioscorea polystachya,Dioscorea villosa,Shan Yao,Wild Yam,Chinese Yams,Shan Yaos,Wild Yams,Yam, Chinese,Yam, Wild,Yams,Yams, Chinese,Yams, Wild,Yao, Shan,Yaos, Shan

Related Publications

C M Liao, and J Li, and X H Liu, and Y S Zhang
September 2006, Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials,
C M Liao, and J Li, and X H Liu, and Y S Zhang
July 2017, Food chemistry,
C M Liao, and J Li, and X H Liu, and Y S Zhang
April 2017, Food chemistry,
C M Liao, and J Li, and X H Liu, and Y S Zhang
May 2020, Food chemistry,
C M Liao, and J Li, and X H Liu, and Y S Zhang
January 2009, Cryo letters,
C M Liao, and J Li, and X H Liu, and Y S Zhang
August 2017, Food chemistry,
C M Liao, and J Li, and X H Liu, and Y S Zhang
June 1993, Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica,
C M Liao, and J Li, and X H Liu, and Y S Zhang
March 2015, International journal of molecular sciences,
C M Liao, and J Li, and X H Liu, and Y S Zhang
April 2018, Food chemistry,
C M Liao, and J Li, and X H Liu, and Y S Zhang
November 2003, Yi chuan = Hereditas,
Copied contents to your clipboard!