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A rapid ELISA method has been developed to quantitate myoglobin in serum. An antigen capture enzyme-linked immunoassay with IgG1 mouse monoclonal antibodies produced by cell clones MGB20-4A1.1 and MGB20-3C1.2 were used for myoglobin detection. These monoclonal antibodies are specific for different epitopes of the myoglobin molecule. Monoclonal antibodies from the hybridoma clone MGB20-4A1.1 were adsorbed to microtiter plate wells. The plates were washed with PBS containing 0.05% Tween 20 and then 20 microliter of standard serum or serum of patients and 200 microliter of peroxidase labeled monoclonal antibodies MGB20-3C1.2 were added to each well. Plates were incubated for 90 min at 37 degrees C and enzyme activity was determined using o-phenylenediamine as a substrate. The ELISA assay described is a rapid and sensitive procedure to assess the quantity of myoglobin within the range 2-1000 ng/ml serum. 120 samples can be tested in 3 h.
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
January 1986,
Acta microbiologica Hungarica,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
July 1990,
Veterinary immunology and immunopathology,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
March 2012,
Journal of virological methods,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
January 2001,
Methods in molecular biology (Clifton, N.J.),
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
September 2020,
Microorganisms,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
June 1989,
Journal of immunological methods,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
June 2002,
Hybridoma and hybridomics,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
September 2000,
Clinica chimica acta; international journal of clinical chemistry,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
October 1998,
Clinical chemistry,
E I Juronen, and
M H Viikmaa, and
A V Mikelsaar
October 2008,
The new microbiologica,
