Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct. 2014

Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
Centro de Desenvolvimento Tecnológico em Saúde, CTDS-Fiocruz. Avenida Brasil 4036, Prédio da Expansão, 8o andar, Sala 814, Rio de Janeiro, RJ, 21040-361, Brazil.

OBJECTIVE Glucuronoxylomannan (GXM) is the major polysaccharide component of Cryptococcus neoformans. We evaluated in this study whether GXM fractions of different molecular masses were functionally distinct. METHODS GXM samples isolated from C. neoformans cultures were fractionated to generate polysaccharide preparations differing in molecular mass. These fractions were used in experiments focused on the association of GXM with cell wall components of C. neoformans, as well as on the interaction of the polysaccharide with host cells. CONCLUSIONS GXM fractions of variable molecular masses bound to the surface of a C. neoformans acapsular mutant in a punctate pattern that is in contrast to the usual annular pattern of surface coating observed when GXM samples containing the full molecular mass range were used. The polysaccharide samples were also significantly different in their ability to stimulate cytokine production by host cells. Our findings indicate that GXM fractions are functionally distinct depending on their mass.

UI MeSH Term Description Entries
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D011134 Polysaccharides Long chain polymeric CARBOHYDRATES composed of MONOSACCHARIDES linked by glycosidic bonds. Glycan,Glycans,Polysaccharide
D011485 Protein Binding The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments. Plasma Protein Binding Capacity,Binding, Protein
D003453 Cryptococcosis Fungal infection caused by genus CRYPTOCOCCUS. C gattii Infection,C neoformans Infection,C. gattii Infection,C. neoformans Infection,Cryptococcus Infection,Cryptococcus Infections,Cryptococcus gattii Infection,Torulosis,Cryptococcus neoformans Infection,C gattii Infections,C neoformans Infections,C. gattii Infections,C. neoformans Infections,Cryptococcoses,Cryptococcus gattii Infections,Cryptococcus neoformans Infections,Infection, C gattii,Infection, C neoformans,Infection, C. gattii,Infection, C. neoformans,Infection, Cryptococcus,Infection, Cryptococcus gattii,Infection, Cryptococcus neoformans,Infections, C gattii,Infections, C. neoformans,Toruloses
D003455 Cryptococcus neoformans A species of the fungus CRYPTOCOCCUS. Its teleomorph is Filobasidiella neoformans. Blastomyces neoformans,Debaryomyces neoformans,Filobasidiella neoformans,Lipomyces neoformans,Saccharomyces neoformans,Torula neoformans,Torulopsis neoformans,Cryptococcus neoformans var. grubii
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013347 Subcellular Fractions Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163) Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine

Related Publications

Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
September 1994, Infection and immunity,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
August 2003, Medical mycology,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
September 1996, Infection and immunity,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
November 1993, Carbohydrate research,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
July 1995, Antimicrobial agents and chemotherapy,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
January 2020, Frontiers in cellular and infection microbiology,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
April 1982, Mycopathologia,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
January 1991, Infection and immunity,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
January 2012, Microbial pathogenesis,
Priscila C Albuquerque, and Fernanda L Fonseca, and Fabianno F Dutra, and Marcelo T Bozza, and Susana Frases, and Arturo Casadevall, and Marcio L Rodrigues
December 1989, Infection and immunity,
Copied contents to your clipboard!