-transparent.png){kind=logo}
Th cell-mediated rapid recognition of foreign Ag and the Ia molecule was studied using azobenzenearsonate-L-tyrosine (ABA-L-tyrosine)-specific Th cells (I-Ak restricted), foreign Ag (ABA-L-tyrosine), and APC (H-2k). Initial transmembrane signals in Th cell hybridomas (2-45-12) and in Th cell lines (A24-17 or A33-7) were monitored by stopped-flow fluorometry with fluorescent probes. It was found that Th cells recognized foreign Ag within 1 s at 25 degrees C on the APC (B10.BR spleen cells or L cells into which I-Ak genes were transferred). Recognition of foreign Ag and the Ia molecule was shown to deliver the initial signals to Th cell hybridomas and T cell lines. First, Th cells had membrane fluidity increased and then calcium was transported from the external medium into the T cells. The initial transmembrane signals to Th cell hybridomas were inhibited by the addition of an anti-I-Ak mAb. None of the initial signals were observed in the absence of either specific foreign Ag or APC.
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
January 1986,
Nature,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
January 1987,
Annales de l'Institut Pasteur. Immunology,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
September 1989,
Immunology,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
December 1986,
Cell,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
June 1987,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
May 2013,
Journal of visualized experiments : JoVE,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
December 1990,
Journal of immunology (Baltimore, Md. : 1950),
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
December 2021,
Proceedings of the National Academy of Sciences of the United States of America,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
February 1988,
Seikagaku. The Journal of Japanese Biochemical Society,
N Utsunomiya, and
M Nakanishi, and
Y Arata, and
T Yasuda, and
S Saito, and
K Koyama, and
T Tadakuma
August 1989,
Nature,
