-transparent.png){kind=logo}
Microfluidic, marker-free isolation of circulating tumor cells from blood samples. 2014
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
1] Department of Surgery and Center for Engineering in Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [2].
The ability to isolate and analyze rare circulating tumor cells (CTCs) has the potential to further our understanding of cancer metastasis and enhance the care of cancer patients. In this protocol, we describe the procedure for isolating rare CTCs from blood samples by using tumor antigen-independent microfluidic CTC-iChip technology. The CTC-iChip uses deterministic lateral displacement, inertial focusing and magnetophoresis to sort up to 10⁷ cells/s. By using two-stage magnetophoresis and depletion antibodies against leukocytes, we achieve 3.8-log depletion of white blood cells and a 97% yield of rare cells with a sample processing rate of 8 ml of whole blood/h. The CTC-iChip is compatible with standard cytopathological and RNA-based characterization methods. This protocol describes device production, assembly, blood sample preparation, system setup and the CTC isolation process. Sorting 8 ml of blood sample requires 2 h including setup time, and chip production requires 2-5 d.
Related Publications
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
October 2019,
Cytometry. Part A : the journal of the International Society for Analytical Cytology,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
January 2021,
Sovremennye tekhnologii v meditsine,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
January 2017,
Methods in molecular biology (Clifton, N.J.),
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
January 2013,
Biomicrofluidics,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
June 2012,
Journal of visualized experiments : JoVE,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
December 2023,
Electrophoresis,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
July 2019,
Biomicrofluidics,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
January 2017,
NPJ precision oncology,
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
January 2019,
Methods in molecular biology (Clifton, N.J.),
Nezihi Murat Karabacak, and
Philipp S Spuhler, and
Fabio Fachin, and
Eugene J Lim, and
Vincent Pai, and
Emre Ozkumur, and
Joseph M Martel, and
Nikola Kojic, and
Kyle Smith, and
Pin-i Chen, and
Jennifer Yang, and
Henry Hwang, and
Bailey Morgan, and
Julie Trautwein, and
Thomas A Barber, and
Shannon L Stott, and
Shyamala Maheswaran, and
Ravi Kapur, and
Daniel A Haber, and
Mehmet Toner
October 2017,
Lab on a chip,
