BIOMAT Database Logo BIOMAT Database Logo

Analysis of the molecular specificities of anti-class II monoclonal antibodies by using L cell transfectants expressing HLA class II molecules. 1988

E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Veterans Administration Medical Center, Iowa City 52240.

Expressible HLA class II alpha- and beta-chain cDNA were used for DNA-mediated gene transfer to produce L cell transfectants expressing single types of human class II molecules. Cloned transfectants expressing nine different class II molecules were isolated: DR alpha: DR1 beta I, DR alpha: DR4 beta I, DR alpha: DR5 beta I, DR alpha: DR5 beta III (DRw52), DR alpha: DR7 beta I, DR alpha: DR4/7 beta IV (DRw53), DQ7 alpha: DQw2 beta, DQ7 alpha: DQw3 beta, and DPw4 alpha: DPw4 beta. These class II-expressing transfectants were used to analyze by flow cytometry the molecular specificities of 20 anti-class II mAb. These analyes indicate that some mAb are more broadly reactive than was previously thought based on immunochemical studies. In contrast, the narrow molecular specificities of other anti-class II mAb were confirmed by this approach. Transfectants expressing human class II molecules should be valuable reagents for studies of B cell and T cell defined epitopes on these molecules.

UI MeSH Term Description Entries
D007739 L Cells A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS. Earle's Strain L Cells,L Cell Line,L Cells (Cell Line),L-Cell Line,L-Cells,L-Cells, Cell Line,L929 Cell Line,L929 Cells,NCTC Clone 929 Cells,NCTC Clone 929 of Strain L Cells,Strain L Cells,Cell Line L-Cell,Cell Line L-Cells,Cell Line, L,Cell Line, L929,Cell Lines, L,Cell, L,Cell, L (Cell Line),Cell, L929,Cell, Strain L,Cells, L,Cells, L (Cell Line),Cells, L929,Cells, Strain L,L Cell,L Cell (Cell Line),L Cell Lines,L Cell, Strain,L Cells, Cell Line,L Cells, Strain,L-Cell,L-Cell Lines,L-Cell, Cell Line,L929 Cell,Strain L Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006681 HLA-D Antigens Human immune-response or Class II antigens found mainly, but not exclusively, on B-lymphocytes and produced from genes of the HLA-D locus. They are extremely polymorphic families of glycopeptides, each consisting of two chains, alpha and beta. This group of antigens includes the -DR, -DQ and -DP designations, of which HLA-DR is most studied; some of these glycoproteins are associated with certain diseases, possibly of immune etiology. Antigens, HLA-D,Class II Human Antigens,HLA-Dw Antigens,Human Class II Antigens,Ia-Like Antigens, Human,Immune Response-Associated Antigens, Human,Immune-Associated Antigens, Human,Immune-Response Antigens, Human,HLA-D,HLA-Dw,Immune Response Associated Antigens, Human,Antigens, HLA D,Antigens, HLA-Dw,Antigens, Human Ia-Like,Antigens, Human Immune-Associated,Antigens, Human Immune-Response,HLA D Antigens,HLA Dw Antigens,Human Ia-Like Antigens,Human Immune-Associated Antigens,Human Immune-Response Antigens,Ia Like Antigens, Human,Immune Associated Antigens, Human,Immune Response Antigens, Human
D006682 HLA-DP Antigens A group of the D-related HLA antigens (human) found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases. HLA-PL Antigens,HLA-SB Antigens,HLA-DP,HLA-PL,HLA-SB,Antigens, HLA-DP,Antigens, HLA-PL,Antigens, HLA-SB,HLA DP Antigens,HLA PL Antigens,HLA SB Antigens
D006683 HLA-DQ Antigens A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases. HLA-DC Antigens,HLA-MB Antigens,HLA-DC,HLA-DQ,HLA-DS,HLA-DS Antigens,HLA-LB,HLA-LB Antigens,HLA-MB,Antigens, HLA-DC,Antigens, HLA-DQ,Antigens, HLA-DS,Antigens, HLA-LB,Antigens, HLA-MB,HLA DC Antigens,HLA DQ Antigens,HLA DS Antigens,HLA LB Antigens,HLA MB Antigens
D006684 HLA-DR Antigens A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS. HLA-DR,Antigens, HLA-DR,HLA DR Antigens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000918 Antibody Specificity The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site. Antibody Specificities,Specificities, Antibody,Specificity, Antibody

Related Publications

E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants.
November 1986, Journal of immunology (Baltimore, Md. : 1950),
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants.
April 1995, Animal genetics,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Fine mapping of HLA class II monoclonal antibody specificities using transfected L cells.
January 1990, Immunogenetics,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Generation and characterization of an HLA-DR alpha-specific monoclonal antibody using L-cell transfectants expressing human and mouse class II major histocompatibility dimers.
January 1991, Immunogenetics,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Characterization of monoclonal antibodies directed against HLA class II molecules.
January 1986, Hybridoma,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Fine analysis of recognition sites of monoclonal antibodies, 1B7 and 1E4, using L cell transfectants of recombinant mouse MHC class II genes.
February 1993, European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
THE SPECIFICITY OF ANTI-HLA CLASS II MONOCLONAL ANTIBODIES IN CATTLE.
June 1997, European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
The specificity of anti-HLA class II monoclonal antibodies in cattle.
June 1997, European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Inhibition by anti-HLA class II monoclonal antibodies of monocyte-dependent T cell proliferation induced by monoclonal antibody OKT3.
November 1987, European journal of immunology,
E P Klohe, and R Watts, and M Bahl, and C Alber, and W Y Yu, and R Anderson, and J Silver, and P K Gregersen, and R W Karr
Generation and characterization of murine monoclonal antibodies against HLA Class II molecules.
January 1984, Diagnostic immunology,
Copied contents to your clipboard!