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Uncoupler-inhibitor titrations of ATP-driven reverse electron transfer across the first site of the respiratory chain were performed in isolated rat-liver mitochondria, and the experimental results were compared with the predictions of a simple delocalized chemiosmotic mechanism. The rates of ATP hydrolysis (Jp) and reverse electron transfer (-J0) were measured at different uncoupler (S-13) concentrations, either in the absence or in the presence of rotenone. When the rates -J0 and Jp measured at different uncoupler concentrations were expressed as percentages of the activity at zero uncoupler concentration, it was found that the efficiency of S-13 to uncouple the reverse electron transfer and to stimulate ATP hydrolysis was not significantly changed upon partial inhibition with rotenone. These results are in contrast with data from a study of uncoupler-inhibitor titrations in submitochondrial particles published previously, in which a higher effectiveness of several uncouplers to inhibit ATP-driven reverse electron transfer was observed in the presence of rotenone.
J Sánchez Olavarría, and
A Lambers, and
K van Dam
April 1986,
Biochimica et biophysica acta,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
March 1971,
FEBS letters,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
June 1980,
The Journal of biological chemistry,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
June 1973,
FEBS letters,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
February 1986,
Life sciences,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
December 1973,
Biochimica et biophysica acta,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
June 1979,
Biochimica et biophysica acta,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
January 1982,
Mikrobiologiia,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
February 1970,
The Journal of cell biology,
J Sánchez Olavarría, and
A Lambers, and
K van Dam
February 1996,
Archives of biochemistry and biophysics,
