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The subcellular distribution of 5-hydroxytryptamine (5-HT) and substance P (SP) was investigated in rat ventral spinal cord using differential and density gradient centrifugation. 5-HT was determined by high performance liquid chromatography (HPLC) and SP by radioimmunoassay (RIA). Both substances were found to be stored within synaptosomes at high densities. After partial lysis of the synaptosomes, 5-HT was also recovered in low density fractions, while substance P was found also in a fraction of intermediate density. The results indicate that within synaptosomes from ventral spinal cord, most of the 5-HT is stored in small synaptic vesicles, while SP is only stored in large synaptic vesicles. A minor part of the 5-HT may in addition also be stored in large vesicles, as previously suggested by ultrastructural studies.
G Fried, and
J Franck, and
E Brodin
January 1982,
Comparative biochemistry and physiology. C: Comparative pharmacology,
G Fried, and
J Franck, and
E Brodin
February 1992,
Neuroscience letters,
G Fried, and
J Franck, and
E Brodin
January 1997,
Journal of neurochemistry,
G Fried, and
J Franck, and
E Brodin
February 1986,
Neuroscience letters,
G Fried, and
J Franck, and
E Brodin
November 1981,
European journal of pharmacology,
G Fried, and
J Franck, and
E Brodin
January 1982,
Histochemistry,
G Fried, and
J Franck, and
E Brodin
June 1966,
Journal of neurochemistry,
G Fried, and
J Franck, and
E Brodin
March 1979,
The Journal of comparative neurology,
G Fried, and
J Franck, and
E Brodin
June 1996,
Neuropharmacology,
G Fried, and
J Franck, and
E Brodin
January 1983,
Anatomischer Anzeiger,
