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The rat renal papillary interstitum which contains abundant proteoglycans is a unique area important in renal function. These proteoglycans were studied ultrastructurally by ruthenium red fixation and staining and phosphate-buffered fixation before and after enzyme digestion. A tissue culture of rat renomedullary interstitial cells, the predominant cell of the renal papillary interstitum, was studied for its ability to synthesize proteoglycans and the proteoglycans were then analyzed. Tissue slices of whole rat renal inner medulla were also evaluated for their synthetic ability. In combination, these studies indicate that the dominant glycosaminoglycan is hyaluronic acid. The tissue culture of rat renal medullary interstitial cells synthesized glycosaminoglycans and on analysis, hyaluronic acid was found to be the chief glycosaminoglycan secreted by the renomedullary interstitial cells. Combined with the removal of the proteoglycans from tissue by leech hyaluronidase and testicular hyaluronidase, this suggests that the dominant glycosaminoglycan is hyaluronic acid. Hyaluronic acid is also synthesized by the intact papilla confirming the findings with the tissue culture. However, in addition, sulfated glycosaminoglycans were also synthesized by the intact papilla, presumably the product of the noninterstitial components of the papilla.
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
January 1986,
Minerva urologica e nefrologica = The Italian journal of urology and nephrology,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
June 1977,
British journal of haematology,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
September 1968,
Journal of anatomy,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
December 1982,
Archives of ophthalmology (Chicago, Ill. : 1960),
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
December 1986,
Experimental and molecular pathology,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
January 1970,
Revue europeenne d'etudes cliniques et biologiques. European journal of clinical and biological research,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
January 1972,
The Journal of clinical investigation,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
July 1985,
Blood,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
January 1987,
Transactions of the American Clinical and Climatological Association,
J A Pitcock, and
H Lyons, and
P S Brown, and
W A Rightsel, and
E E Muirhead
February 1983,
Blood,
