Antioxidant activity of bovine casein hydrolysates produced by Ficus carica L.-derived proteinase. 2014

Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
Department of Life Sciences, University of Limerick, Limerick, Ireland; Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE, Università di Padova, Legnaro, Padova, Italy.

A Ficus carica L. latex proteinase preparation was investigated for its ability to produce antioxidant hydrolysates/peptides from bovine casein (CN). The Oxygen Radical Absorbance Capacity (ORAC) values for NaCN and β-CN hydrolysates ranged from 0.06 to 0.18, and from 0.51 to 1.19μmol Trolox equivalents/mg freeze-dried sample, respectively. Gel permeation HPLC showed that the β-CN hydrolysate with a degree of hydrolysis of 21% had 65% of peptide material with a molecular mass <500Da. The RP-UPLC profiles also indicated that β-CN was substantially hydrolysed during the early stages of hydrolysis. Analysis of the 4h β-CN hydrolysate by LC-ESI-MS/MS allowed identification of 8 peptide sequences with potential antioxidant properties.

UI MeSH Term Description Entries
D010084 Oxidation-Reduction A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471). Redox,Oxidation Reduction
D002364 Caseins A mixture of related phosphoproteins occurring in milk and cheese. The group is characterized as one of the most nutritive milk proteins, containing all of the common amino acids and rich in the essential ones. alpha-Casein,gamma-Casein,AD beta-Casein,Acetylated, Dephosphorylated beta-Casein,Casein,Casein A,K-Casein,Sodium Caseinate,alpha(S1)-Casein,alpha(S1)-Casein A,alpha(S1)-Casein B,alpha(S1)-Casein C,alpha(S2)-Casein,alpha-Caseins,beta-Casein,beta-Caseins,epsilon-Casein,gamma-Caseins,kappa-Casein,kappa-Caseins,AD beta Casein,Caseinate, Sodium,K Casein,alpha Casein,alpha Caseins,beta Casein,beta Caseins,beta-Casein Acetylated, Dephosphorylated,beta-Casein, AD,epsilon Casein,gamma Casein,gamma Caseins,kappa Casein,kappa Caseins
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000975 Antioxidants Naturally occurring or synthetic substances that inhibit or retard oxidation reactions. They counteract the damaging effects of oxidation in animal tissues. Anti-Oxidant,Antioxidant,Antioxidant Activity,Endogenous Antioxidant,Endogenous Antioxidants,Anti-Oxidant Effect,Anti-Oxidant Effects,Anti-Oxidants,Antioxidant Effect,Antioxidant Effects,Activity, Antioxidant,Anti Oxidant,Anti Oxidant Effect,Anti Oxidant Effects,Anti Oxidants,Antioxidant, Endogenous,Antioxidants, Endogenous
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D030681 Ficus A plant genus of the family MORACEAE. It is the source of the familiar fig fruit and the latex from this tree contains FICAIN. Figs,Rubberplant,Fig,Rubberplants

Related Publications

Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
December 2023, Journal of the science of food and agriculture,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
July 2020, Scientific reports,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
January 2012, Natural product research,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
May 2002, Cutis,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
June 2009, Plant foods for human nutrition (Dordrecht, Netherlands),
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
August 2023, Journal of biomolecular structure & dynamics,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
September 2018, Physiology and molecular biology of plants : an international journal of functional plant biology,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
July 2019, Cellular and molecular biology (Noisy-le-Grand, France),
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
October 2019, Journal of traditional and complementary medicine,
Giovanna Di Pierro, and Martina B O'Keeffe, and Alexey Poyarkov, and Giovanna Lomolino, and Richard J FitzGerald
November 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
Copied contents to your clipboard!