Biomaterial Database

Acceleration of anterograde axonal transport in cat sciatic nerve by diisopropyl phosphorofluoridate. 1989

C D Carrington, and D M Lapadula, and M B Abou-Donia

Department of Pharmacology, Duke University Medical Center, Durham, NC 27710.

The effect of a neurotoxic dose of 5.0 mg/kg, s.c. diisopropyl phosphorofluoridate (DFP) on anterograde transport of 35S-methionine labeled proteins in cat peripheral nerve was studied. Seven days after dosing, after 24 h of flow there was 48% less radioactivity in the distal portion of the nerve. A lesser effect was also found at 4 and 14 days after dosing. After 10 or 14 h of flow, the height of the crest was unchanged, but the distance of the crest from the ganglia was greater in the DFP-treated animals. These experiments indicate that DFP treatment accelerates fast anterograde transport.

UI	MeSH Term	Description	Entries
D007531	Isoflurophate	A di-isopropyl-fluorophosphate which is an irreversible cholinesterase inhibitor used to investigate the NERVOUS SYSTEM.	DFP,Diisopropylfluorophosphate,Fluostigmine,Bis(1-methylethyl) Phosphorofluoridate,Di-isopropylphosphorofluoridate,Diisopropylphosphofluoridate,Dyflos,Floropryl,Fluorostigmine,Di isopropylphosphorofluoridate
D008297	Male		Males
D009419	Nerve Tissue Proteins		Proteins, Nerve Tissue,Tissue Proteins, Nerve
D002415	Cats	The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)	Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001370	Axonal Transport	The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)	Axoplasmic Flow,Axoplasmic Transport,Axoplasmic Streaming,Axonal Transports,Axoplasmic Flows,Axoplasmic Transports,Streaming, Axoplasmic,Transport, Axonal,Transport, Axoplasmic,Transports, Axonal,Transports, Axoplasmic
D012584	Sciatic Nerve	A nerve which originates in the lumbar and sacral spinal cord (L4 to S3) and supplies motor and sensory innervation to the lower extremity. The sciatic nerve, which is the main continuation of the sacral plexus, is the largest nerve in the body. It has two major branches, the TIBIAL NERVE and the PERONEAL NERVE.	Nerve, Sciatic,Nerves, Sciatic,Sciatic Nerves