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OBJECTIVE To establish chemiluminescence enzyme immunoassay (CLEIA) for quantitative detection of procollagen III N-terminal peptide (P III NP) in serum. METHODS A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of P III NP as the catalytic enzyme and the luminol as the luminescence reagent. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as linear range, sensitivity, specificity, stability and so on. The CLEIA was compared with imported ELISA kits, by detecting clinical serum. RESULTS The linear range was 0.8-85 ng/ml. The detection limit was 0.5 ng/ml. Inter-assay and intra-assay RSD were both less than 10%. The recoveries of three different spiked concentration samples were 96.2%, 91.2% and 101.1%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.99 and RSD lower than 6%. The detected results of clinical sera with CLEIA closely corresponded to those with imported ELISA. CONCLUSIONS Established CLEIA for quantity determination of serum P III NP has high accuracy, sensitivity and repeatability.
Jia Liu, and
Yan-Qing Feng, and
Yong-Ji Song, and
Jun Hou, and
Lin Chen, and
Jing Zhao, and
Ai-Xia Liu, and
Jing-Xia Guo, and
Jun Xu, and
Hong-Shan Wei, and
Bo-An Li
December 2021,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
Jia Liu, and
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Bo-An Li
January 1992,
Experimental lung research,
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Jun Hou, and
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Bo-An Li
April 1990,
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Jia Liu, and
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July 1987,
Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
Jia Liu, and
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June 1987,
Experimental and molecular pathology,
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Frontiers in pediatrics,
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Jia Liu, and
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