Biomaterial Database

[Analyses of HLA antigens using cytotoxic T lymphocyte clones]. 1988

Y Tajima

Department of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.

In order to investigate the structural polymorphism of DQ antigens, especially those associated with DRw8, allospecific cytotoxic T lymphocyte (CTL) clones were produced. Three informative human CTL clones, TGBE-11, TGAF-3 and TED-2 were established. The first two CTL clones were generated against DRw8/DQWa typed B-lymphoblastoid cell line (LCL), GI and the third against DR4/DQw3 typed B- LCL,ER. TGBE-11 lysed not only some DRw8 but also DR1 positive LCLs. However, it did not react with the DRw8 positive LCLs associated with DQw1. Cytotoxicity of this CTL clone was inhibited by anti-DR monoclonal antibody (MoAb). These results indicated that DRw8 is not homogenous and that the DRw8 molecule has the common determinant with DR1. On the other hand, TGAF-3 and TED-2 reacted with DRw8/DQWa typed LCLs without any cross reaction with DR4/DQWa typed LCLs. Since their reactivity was inhibited by anti-DQ MoAbs, they were thought to discriminate DQWa of DRw8 LCLs from that of DR4 LCLs. This result was confirmed by the fact that 2-D profiles of HU-46 exhibited different electrophoretic patterns of DQWa between DR4 and DRw8. Moreover, further splits of DQWa were suggested because the reactivity of TGAF-3 and that of TED-2 were mutually exclusive against DRw8/DQWa panels. By using these two CTL clones, Dw8/DRw8 could be subdivided into three groups, Dw8.1, Dw8.2 and Dw8.3, corresponding to D-typing. In other words, Dw8.1 was defined by TGAF-3, Dw8.2 by TED-2 and Dw8.3 by negative reaction with both CTL clones.(ABSTRACT TRUNCATED AT 250 WORDS)

UI	MeSH Term	Description	Entries
D002999	Clone Cells	A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)	Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D006680	HLA Antigens	Antigens determined by leukocyte loci found on chromosome 6, the major histocompatibility loci in humans. They are polypeptides or glycoproteins found on most nucleated cells and platelets, determine tissue types for transplantation, and are associated with certain diseases.	Human Leukocyte Antigen,Human Leukocyte Antigens,Leukocyte Antigens,HL-A Antigens,Antigen, Human Leukocyte,Antigens, HL-A,Antigens, HLA,Antigens, Human Leukocyte,Antigens, Leukocyte,HL A Antigens,Leukocyte Antigen, Human,Leukocyte Antigens, Human
D006683	HLA-DQ Antigens	A group of the D-related HLA antigens found to differ from the DR antigens in genetic locus and therefore inheritance. These antigens are polymorphic glycoproteins comprising alpha and beta chains and are found on lymphoid and other cells, often associated with certain diseases.	HLA-DC Antigens,HLA-MB Antigens,HLA-DC,HLA-DQ,HLA-DS,HLA-DS Antigens,HLA-LB,HLA-LB Antigens,HLA-MB,Antigens, HLA-DC,Antigens, HLA-DQ,Antigens, HLA-DS,Antigens, HLA-LB,Antigens, HLA-MB,HLA DC Antigens,HLA DQ Antigens,HLA DS Antigens,HLA LB Antigens,HLA MB Antigens
D006684	HLA-DR Antigens	A subclass of HLA-D antigens that consist of alpha and beta chains. The inheritance of HLA-DR antigens differs from that of the HLA-DQ ANTIGENS and HLA-DP ANTIGENS.	HLA-DR,Antigens, HLA-DR,HLA DR Antigens
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000939	Epitopes	Sites on an antigen that interact with specific antibodies.	Antigenic Determinant,Antigenic Determinants,Antigenic Specificity,Epitope,Determinant, Antigenic,Determinants, Antigenic,Specificity, Antigenic
D013602	T-Lymphocytes, Cytotoxic	Immunized T-lymphocytes which can directly destroy appropriate target cells. These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. The lytic phenomenon is sometimes referred to as cell-mediated lympholysis (CML). These CD8-positive cells are distinct from NATURAL KILLER CELLS and NATURAL KILLER T-CELLS. There are two effector phenotypes: TC1 and TC2.	Cell-Mediated Lympholytic Cells,Cytotoxic T Cells,Cytotoxic T Lymphocyte,Cytotoxic T-Lymphocytes,TC1 Cell,TC1 Cells,TC2 Cell,TC2 Cells,Cell Mediated Lympholytic Cells,Cell, Cell-Mediated Lympholytic,Cell, TC1,Cell, TC2,Cell-Mediated Lympholytic Cell,Cytotoxic T Cell,Cytotoxic T Lymphocytes,Cytotoxic T-Lymphocyte,Lymphocyte, Cytotoxic T,Lympholytic Cell, Cell-Mediated,Lympholytic Cells, Cell-Mediated,T Cell, Cytotoxic,T Lymphocyte, Cytotoxic,T Lymphocytes, Cytotoxic,T-Lymphocyte, Cytotoxic
D015798	HLA-DR1 Antigen	An HLA-DR antigen associated with HLA-DRB1 CHAINS that are encoded by DRB1*01 alleles.	HLA-DR1,Antigen, HLA-DR1,HLA DR1 Antigen
D059810	HLA-DR Serological Subtypes	HLA-DR antigen subtypes that have been classified according to their affinity to specific ANTIBODIES. The DNA sequence analyses of HLA-DR ALPHA-CHAINS and HLA-DR BETA-CHAINS has for the most part revealed the specific alleles that are responsible for each serological subtype.	HLA DR Serological Subtypes,Serological Subtypes, HLA-DR,Subtypes, HLA-DR Serological