Reconstitution of the Na+/H+ antiporter: a new method for the determination of H+ efflux from Na+/H+ antiporter-reconstituted vesicles. 1988

S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.

A Na+/H+ antiporter was solubilized with octyl-beta-D-glucopyranoside from rat renal brush border membrane and reconstituted in egg phosphatidylcholine liposomes. The activity of the antiporter was determined by a new method. Reconstitution was performed in a solution containing a pH-sensitive fluorescence dye, fluorescein isothiocyanate-dextran (FITC-dextran), at pH 5.5. The reconstituted vesicles, encapsulating FITC-dextran, were incubated at pH 8.0, and the intravesicular pH (pHi) was determined by monitoring fluorescence change of the dye. An increase in the fluorescence intensity which corresponded to an increase in pHi was observed. The rate of the increase was dependent on the concentration of the extravesicular Na+, and the efflux of H+ in the presence of 150 mM sodium gluconate was suppressed by the addition of amiloride. These results show that the Na+/H+ antiporter was functionally reconstituted and that its activity can be determined simply by the use of FITC-dextran.

UI MeSH Term Description Entries
D007474 Ion Exchange Reversible chemical reaction between a solid, often one of the ION EXCHANGE RESINS, and a fluid whereby ions may be exchanged from one substance to another. This technique is used in water purification, in research, and in industry. Exchange, Ion
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D008871 Microvilli Minute projections of cell membranes which greatly increase the surface area of the cell. Brush Border,Striated Border,Border, Brush,Border, Striated,Borders, Brush,Borders, Striated,Brush Borders,Microvillus,Striated Borders
D011522 Protons Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion. Hydrogen Ions,Hydrogen Ion,Ion, Hydrogen,Ions, Hydrogen,Proton
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D003911 Dextrans A group of glucose polymers made by certain bacteria. Dextrans are used therapeutically as plasma volume expanders and anticoagulants. They are also commonly used in biological experimentation and in industry for a wide variety of purposes. Dextran,Dextran 40,Dextran 40000,Dextran 70,Dextran 75,Dextran 80,Dextran B-1355,Dextran B-1355-S,Dextran B1355,Dextran B512,Dextran Derivatives,Dextran M 70,Dextran T 70,Dextran T-40,Dextran T-500,Hemodex,Hyskon,Infukoll,Macrodex,Polyglucin,Promit,Rheodextran,Rheoisodex,Rheomacrodex,Rheopolyglucin,Rondex,Saviosol,Dextran B 1355,Dextran B 1355 S,Dextran T 40,Dextran T 500
D005452 Fluoresceins A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays. Tetraiodofluorescein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012964 Sodium A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23. Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23

Related Publications

S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
September 1985, The Journal of biological chemistry,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
January 1990, Life sciences,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
April 1989, The Journal of biological chemistry,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
August 1994, Archives of biochemistry and biophysics,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
October 1989, The American journal of physiology,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
August 1990, Biochemical and biophysical research communications,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
September 1992, Nihon rinsho. Japanese journal of clinical medicine,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
January 1981, Biochimica et biophysica acta,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
April 1991, The Journal of biological chemistry,
S Shibamoto, and S Yoshida, and N Oku, and M Hayakawa, and T Hori, and F Ito
May 1988, Plant physiology,
Copied contents to your clipboard!