BIOMAT Database Logo BIOMAT Database Logo

OmpA and OmpC are critical host factors for bacteriophage Sf6 entry in Shigella. 2014

Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA; Department of Chemistry & Biochemistry, University of California, San Diego, La Jolla, CA, 92093, USA.

Despite being essential for successful infection, the molecular cues involved in host recognition and genome transfer of viruses are not completely understood. Bacterial outer membrane proteins A and C co-purify in lipid vesicles with bacteriophage Sf6, implicating both outer membrane proteins as potential host receptors. We determined that outer membrane proteins A and C mediate Sf6 infection by dramatically increasing its rate and efficiency. We performed a combination of in vivo studies with three omp null mutants of Shigella flexneri, including classic phage plaque assays and time-lapse fluorescence microscopy to monitor genome ejection at the single virion level. Cryo-electron tomography of phage 'infecting' outer membrane vesicles shows the tail needle contacting and indenting the outer membrane. Lastly, in vitro ejection studies reveal that lipopolysaccharide and outer membrane proteins are both required for Sf6 genome release. We conclude that Sf6 phage entry utilizes either outer membrane proteins A or C, with outer membrane protein A being the preferred receptor.

UI MeSH Term Description Entries
D008070 Lipopolysaccharides Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed) Lipopolysaccharide,Lipoglycans
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D001425 Bacterial Outer Membrane Proteins Proteins isolated from the outer membrane of Gram-negative bacteria. OMP Proteins,Outer Membrane Proteins, Bacterial,Outer Membrane Lipoproteins, Bacterial
D001435 Bacteriophages Viruses whose hosts are bacterial cells. Phages,Bacteriophage,Phage
D012763 Shigella flexneri A bacterium which is one of the etiologic agents of bacillary dysentery (DYSENTERY, BACILLARY) and sometimes of infantile gastroenteritis.
D014771 Virion The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos. Virus Particle,Viral Particle,Viral Particles,Particle, Viral,Particle, Virus,Particles, Viral,Particles, Virus,Virions,Virus Particles
D016679 Genome, Viral The complete genetic complement contained in a DNA or RNA molecule in a virus. Viral Genome,Genomes, Viral,Viral Genomes
D055032 Electron Microscope Tomography A tomographic technique for obtaining 3-dimensional images with transmission electron microscopy. Electron Tomography,Tomography, Electron,Tomography, Electron Microscope,EM Tomography,Electron Microtomography,STEM Tomography,Scanning Transmission Electron Microscopy Tomography,TEM Tomography,Transmission Electron Microscopy Tomography,Transmission Electron Microtomography,Electron Microtomography, Transmission,Microscope Tomography, Electron,Microtomography, Electron,Microtomography, Transmission Electron,Tomography, EM,Tomography, STEM,Tomography, TEM

Related Publications

Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
The host outer membrane proteins OmpA and OmpC are associated with the Shigella phage Sf6 virion.
January 2011, Virology,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Interaction between bacteriophage Sf6 and Shigella flexner.
July 1978, Journal of virology,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Bacteriophage Sf6 host range mutant that infects Shigella flexneri serotype 2a2 strains.
April 2022, FEMS microbiology letters,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens.
August 2018, Viruses,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Key residues of S. flexneri OmpA mediate infection by bacteriophage Sf6.
May 2015, Journal of molecular biology,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Topology and identification of critical residues of the O-acetyltransferase of serotype-converting bacteriophage, SF6, of Shigella flexneri.
October 2008, Biochemical and biophysical research communications,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Host-controlled variation of Shigella bacteriophage.
August 1954, Japanese journal of medical science & biology,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
NRP2 and CD63 Are Host Factors for Lujo Virus Cell Entry.
November 2017, Cell host & microbe,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
The chromosome of Shigella flexneri bacteriophage Sf6: complete nucleotide sequence, genetic mosaicism, and DNA packaging.
May 2004, Journal of molecular biology,
Kristin N Parent, and Marcella L Erb, and Giovanni Cardone, and Katrina Nguyen, and Eddie B Gilcrease, and Natalia B Porcek, and Joe Pogliano, and Timothy S Baker, and Sherwood R Casjens
Host Range Expansion of Shigella Phage Sf6 Evolves through Point Mutations in the Tailspike.
August 2022, Journal of virology,
Copied contents to your clipboard!